雷公藤多苷对糖尿病肾病大鼠肾脏炎性损伤的保护作用  

Protective effect of tripterygium wilfordii polyglycoside on inflammatory injury kidney of rats with diabetic nephropathy

作　　者：芦琨[1] 刘国玲[1] 沈永杰[1] 宋玮[1] 李宜川[1] 

机构地区：[1]商丘医学高等专科学校生物化学与分子生物学教研室,商丘476100

出　　处：《中药药理与临床》2015年第3期93-96,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省教育厅自然科学研究计划项目(2011C310003)

摘　　要：目的:观察雷公藤多苷对糖尿病肾病大鼠白细胞介素1β(IL-1β)、白细胞介素17(IL-17)、干扰素γ(IFN-γ)和单核细胞趋化蛋白-1(MCP-1)等炎性细胞因子表达的影响,讨论其对糖尿病肾病大鼠肾脏炎症损伤的改善作用,分析其可能的治疗机制。方法:将85只雄性SD大鼠分为正常对照组(n=13)和模型组(n=72),采用腹腔注射链脲佐菌素(STZ)的方法建立糖尿病肾病大鼠模型,其中70只大鼠造模成功。将其随机分为模型组、雷公藤多苷治疗组(4.5 mg/kg、9 mg/kg和18 mg/kg)以及罗格列酮(0.4mg/kg)组。末次给药后,检测大鼠血糖(BG)、血甘油三酯(TG)、血总胆固醇(TC)、尿素氮(BUN)、血肌酐(Scr)和24 h尿蛋白量(24 h UAER);放射免疫分析法测定血清中IL-1β水平,酶联免疫吸附法测定各组血清IL-17、IFN-γ的水平;免疫组织化学染色检测肾组织中MCP-1的表达;HE染色观察大鼠肾脏病理变化。结果:与模型组相比,雷公藤多苷9 mg/kg组和18 mg/kg组大鼠的一般情况明显好转,BG、TG、TC、BUN、Scr、24 h UAER的水平显著下降;雷公藤多苷18 mg/kg组大鼠的IL-1β、IL-17、IFN-γ和肾组织中MCP-1的表达明显下调,肾脏病理变化明显减轻。结论:雷公藤多苷可能是通过降低血清及肾脏炎性因子的水平,延缓肾脏的损伤,从而对肾脏起到一定程度的保护作用。Objective ：To observe the protective effect of tripterygium wilfordii polyglycoside （TWP） on the expression of interleukin-113 （IL-113）, interleukin-17（ IL-17）, Interferon-3,（ IFN-3,） and monocyte chemotactic protein-1 （MCP-1） in diabetic nephropathy rats, and to investigate the protective effect on inflammatory injury renal. Methods： 85 male SD rats were randomly divided into normal control group （ n = 13 ） anddiabetic nephropathy（DN） model group （n = 72）. The DN model was induced by intraperitoneal injection of streptozotocin（STZ）. 70 DN model rats were randomly divided into model control group, drug TWP groups（4. 5 mg/kg,9 mg/kg and 18 mg/kg） and rosiglitazone （0.4 mg/kg） group. After the last administration, to detect blood glucose（ BG）, triglycerides （TG）, total cholesterol （TC）, blood urea nitrogen （ BUN）, serum creatinine （ Scr）, and 24 hours urinary albumin excretion rate（UAER） ; The expression of the serum IL-17, IFN-α were ex- amined by ELISA ; IL-1 β were determined by radioimmunoassav. The pathological changes of kidney were observed by HE staining and the expression of MCP-1 in kidney was tested by immunohistochemistry. Results： Compared with the model group, the general status of the rats in TWP （9 mg/kg and 18 mg/kg） were significantly improved; levels of BG, TG, TC, BUN, Scr, 24 h UAER were signiLificantly decreased （P 〈0.01 ）; The expression of IL-1β, IL-17, IFN-y and MCP-1 in kidney of TWP （18 mg/kg） decreased obviously, renal pathological changes were significantly alleviated. Conclusion： TWP could protect the renal tissue by decreasing the inflammatory factors, and inhibiting pathological changes.

关 键 词：雷公藤多苷 糖尿病肾病 炎性损伤 

分 类 号：R285.5[医药卫生—中药学]