银杏酮酯对自然衰老大鼠学习记忆及海马、前额叶皮层c-fos、Caspase-3和Caspase-8表达的影响  

The effects of Ginkgo biloba extract on learning and memory in natural aging rats and the role of c-fos,Caspase-3 and Caspase-8 in the hippocampal and prefrontal cortex

作　　者：郝莉[1] 徐玉英[1] 任秀花[2] 张志雄[3] 高崎 顾慧芬 

机构地区：[1]河南中医学院基础医学院,郑州450052 [2]郑州大学基础医学院,郑州450001 [3]上海中医药大学基础医学院,上海201203 [4]上海杏灵科技药业有限公司,上海200127

出　　处：《中药药理与临床》2015年第3期96-100,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：上海市科委科研计划项目资助(项目批准号09ZR1432100);河南中医学院科研苗圃项目资助(MP2012-03)

摘　　要：目的:探讨银杏酮酯(Ginkgo biloba extract,GBE50)对自然衰老大鼠学习记忆及海马、前额叶皮层c-fos、Caspase-3和Caspase-8表达的影响。方法:21月龄大鼠随机分为模型组、银杏酮酯组和阳性药银杏叶提取物组,1月龄大鼠作为正常组。其中正常组和模型组用1%羧甲基纤维素钠灌胃,银杏酮酯组和银杏叶提取物组分别予100 mg/kg的药物灌胃60天。新物体识别实验检测学习记忆,免疫组织化学方法检测海马CA1区和前额叶皮层c-fos和Caspase-8的蛋白水平,免疫印迹方法检测海马和前额叶皮层Caspase-3蛋白表达。结果:新物体识别实验结果显示:与模型组相比,100mg/kg银杏酮酯和银杏叶提取物可使大鼠的分辨指数和优先指数明显升高。免疫组织化学结果显示:与模型组相比,100mg/kg银杏酮酯和银杏叶提取物可使海马CA1区和前额叶皮层cfos表达增加,Caspase-8表达降低。免疫印迹结果显示:与模型组相比,100mg/kg银杏酮酯和银杏叶提取物组可使海马和前额叶皮层Caspase-3蛋白表达下降。结论:100mg/kg的银杏酮酯可以改善自然衰老大鼠学习记忆缺陷,这种改善可能与上调自然衰老大鼠海马和前额叶皮层c-fos表达,降低Caspase-3和Caspase-8蛋白水平有关。Objective： To investigate effects of Ginkgo biloba extract （GBES0）on learning and memory in natural aging rats, and the role of c-fos, Caspase-3 and Caspase-8 in the hippocampal and prefrontal cortex. Method： 21-month-old rats were randomly divided into three groups： model group, GBES0 group and positive control（extract of Ginkgo biloba, EGB761 ）group, 1 month rats were as normal group. The rats in GBE50 and EGB761 groups received 100 mg/kg GBES0 and EGB761 respectively. The rats in the normal and model groups received solvent （ 1% CMC-Na ＋ ） by intragastric administration for 60 days. Novel object recognition task （NORT） was used to measure learning and memo- ~y; Imunohistochemical staining was used to test e-fos, Caspase-3 and Caspase-8 in the hippocampal and prefrontal cortex. Results： NORT result showed that compared with the model group, the discrimination index and the priority index also increased significantly in the GBE50 and EGB761 groups （P 〈 0, 05 ）. Imunohistochemical staining result showed that e-los increased and Caspase-8 decreased respectively in the hippocampal CA1 and prefrontal cortex after GBE50 and EGB761 （100mg/kg） treatment for 60 days （ P 〈 0. 05 ）. Western blot displayed that compared with the model group, Caspase-3 and Caspase-8 decreased in the hippocampal and prefrontal cortex after GBE50 and EGB761 （100mg/kg） treatment for 60 days （ P 〈 0.05 ）. Conclusion： 100mg/kg GBE50 might improve age-dependent learning and memory deficit, and this might related to the increased expression of c-fos and the decreased expression of Caspase-3 and Caspase-8 in the hippoeampal and prefrontal cortex.

关 键 词：银杏酮酯 自然衰老 学习记忆 C-FOS CASPASE-3 Caspase-8 

分 类 号：R285.5[医药卫生—中药学]