大黄水提物对成年雌性大鼠卵巢Bcl-2和Bax蛋白表达的影响  

Effect of Rhubarb on expressions of Bcl-2 and Bax in rats ovary

作　　者：万慧杰[1] 宋素英[2] 佟继铭[1] 杨宵[1] 卢宁[1] 赵盼盼[1] 张树峰[1] 

机构地区：[1]河北省中药研究与开发重点实验室,承德医学院中药研究所,承德067000 [2]河北联合大学中医学院,唐山063000

出　　处：《中药药理与临床》2015年第3期107-110,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省教育厅(2009301)

摘　　要：目的:观察长期灌胃大黄对成年雌性大鼠卵巢组织中Bcl-2和Bax蛋白表达的影响,分析大黄对卵巢功能的影响及与用药剂量和时间的关系。方法:雌性大鼠90只,随机分为正常对照组、大黄水提物1.0 g/kg给药30d、40d、50d、60d组和2.0 g/kg给药30d、40d、50d、60d组,灌胃给药,采用HE染色法观察卵巢组织病理变化,免疫组化法检测卵巢Bcl-2和Bax蛋白表达情况。结果:大黄水提物1.0 g/kg 60d组和2.0 g/kg 40d、50d、60d组Bcl-2表达显著低于正常对照组,Bax表达显著高于正常对照组;给药50d、60d、1.0 g/kg和2.0 g/kg剂量组相同给药时间比较Bcl-2、Bax表达差异具有统计学意义。相同给药剂量不同给药时间组比较:1.0 g/kg 30d与60d组Bcl-2、Bax表达差异具有统计学意义,2.0 g/kg 30d与50,60d组,40d与50、60d组比较Bcl-2、Bax表达差异具有统计学意义,其他各组Bcl-2、Bax表达差异无统计学意义。结论:长期应用大黄可不同程度地下调卵巢中Bcl-2表达,上调Bax表达,其影响程度与给药剂量及时间呈正相关,推测长期应用大黄可能会通过Bcl-2/Bax途径诱导卵巢颗粒细胞凋亡,进而影响卵巢功能。Objective： To investigate the long-term effect of water extract of Rhubarb （~） on expressions of Bcl-2 and Bax in ovary of female rats and the relationship between the effect and administration dosage and time. Method： Ninety female rats aged eight weeks were randomly di- vided into nine groups ： normal control group , and drug groups which were ig water extract of Rhubarb 1.0 g/kg for 30d, 40d, 50d, 60d and 2.0 g/kg for 30d, 40d, 50d, 60d. The morphological changes in ovary were observed by HE . The expression of the proteins Bcl-2 and Bax on the ovary were detected by immunohistochemistry method. Results： The protein expressions of Bcl-2 and Bax of 1.0 g/kg for 30d,40d,50d groups and 2.0 g/kg 30d group had no significant difference compared with the normal control group, respectively （P 〉 0.05 ）. Whereas the expression of Bcl-2 and Bax in 1.0 g/kg 60d group and 2.0 g/kg 40d,50d,60d groups showed significant deviations from those of the normal group, respectively （ P 〈 0.05 ）. The comParison of the groups when administered different dosage for the same time： the ex- pression of Bcl-2 was lower when adminidtered 2.0 g/kg for 50d ,60d than adminidtered 1.0 g/kg while the expression of Bax showed the op- posite result（ P 〈 0.05）. The comparison of the groups when administered the same dosage for different time： the exPression of Bcl-2 was higher while that of Bax was lower when administered 1.0 g/kg for 60d than 30d （ P 〈 O. 05 ） ; the expression of Bcl-2 decreased while that of Bax increased when adminidstered 50d , 60d than 30d , 40d （ P 〈 0.05）. Conclusion： Rhubarb can down-regulate the expression of Bcl- 2 protein and up-regulate the expression of Bax protein in female rat ovary, which were positively correlated with dosage and time. Bcl-2/Bax system might be involved in Rhubarb to induce ovarian granulose cell apoptosis and results in ovarian dysfunction.

关 键 词：大黄 卵巢 免疫组化 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]