基于黄酮有效组分探讨黄芪、葛根分煎与合煎对糖尿病大鼠降糖降脂作用的影响  

The effect of flavonoids effective components from Astragalus and Kudzu when decocted separately and as a whole on lowering blood glucose and lipid in diabetic rats

作　　者：李艳敏[1,2] 范颖[1,2] 刘烨[1,2] 郝明芬 李新[3] 刘丽[1,2] 訾慧[1,2] 李军[1,2] 

机构地区：[1]辽宁中医药大学方剂学科,辽宁沈阳110847 [2]辽宁中医药大学省部共建中医脏象理论及应用教育部重点实验室,辽宁沈阳110847 [3]辽宁中医药大学数学教研室,辽宁沈阳110847

出　　处：《时珍国医国药》2015年第7期1644-1646,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81273653)

摘　　要：目的探讨不同煎法对黄芪、葛根有效组分(黄酮)干预糖尿病大鼠糖脂代谢的影响及其相关机制。方法 55只SD雄性大鼠按血糖随机分为正常组,模型组,阳性对照组(金芪降糖片),黄芪黄酮+葛根黄酮组(简称分煎组),黄芪葛根汤总黄酮组(简称合煎组)。一次性ip链脲佐菌素(STZ)造模;第30天,分别检测血糖、血清胆固醇(Cholesterol,CHO)、血清甘油三酯(Triglyceride,TG)浓度,肝脏胰岛素(Insulin,INS)、脂联素(Adiponectin,ADPN)、瘦素(Leptin,LEP)、葡萄糖转运体4(glucose transporter 4,GULT4)浓度。结果模型组大鼠血糖、CHO、TG水平显著升高;肝脏INS、ADPN、LEP、GULT4浓度显著降低。与模型组比较,分煎组与合煎组大鼠血糖、血清CHO与TG水平显著降低(P<0.05),两组间差异无统计学意义(P>0.05);在影响糖代谢方面,分煎组INS、GLUT4水平较模型组显著升高,合煎组GLUT4水平较模型组升高明显,且分煎组优于合煎组(P<0.05);在影响相关脂肪因子方面,分煎组与合煎组LEP水平均较模型组显著升高(P<0.05),且两组效果无明显差异(P>0.05)。结论黄芪、葛根分煎与合煎所得到的黄酮类有效组分的降糖降脂整体效应无差异性;但对肝脏INS、GULT4的调节作用,分煎优于合煎。Objective To explore the effect of flavonoids effective components from Astragalus and Kudzu when decocted with different methods on interventing glucose and lipid metabolism in diabetic rats and its related mechanisms.Methods According to random blood glucose,55 SD male rats were divided randomly into 5 groups,such as normal control group,model control group,positive control group（ Jin Qi hypoglycemic tablets）,Astragalus flavonoids Kudzu flavonoids group（ divided decoction group）,and total flavonoids from Huangqi Gegen decoction（ mixed decoction group）.Except the normal control group,the others were injected singlely Streptozocin（ STZ） via intraperitoneal to make diabetic models.After administrating 30 days,blood glucose,serum cholesterol（ CHO） and triglyceride（ TG） were tested,and hepatic insulin（ INS）,glucose transporter 4（ GLUT4）,adiponectin（ ADPN）,and leptin（ LEP） were tested through enzyme- linked immunosorbent assay（ ELISA） Kits as well.Results Compared with the normal group,the glucose,CHO and TG levels elevated,while the levels of hepatic INS,GLUT4,ADPN and LEP were decended.Both divided decoction group and mixed decoction group could reduce levels of blood glucose,serum TG and CHO in diabetic rats（ P〈0.05）,and there were no differences between the two groups（ P〉0.05）.In regulating on glucose metabolism,divided decoction group could significantly increase INS,GLUT4 levels,while mixed decoction group raised GLUT4 level obviously,and the effect of divided decoction group was better than the mixed one（ P〈0.05）.In regulating on the relevant adipokines,both divided decoction group and the mixed one could higher hepatic LEP level identically（ P〈0.05）,however,both of them had no influence on hepatic ADPN level.Conclusion The flavonoids effective components from Astragalus and Kudzu when decocted separately and as a whole showed no significant differences on the effect of lowering blood glucose and lipid,while in regulating on INS and GULT4 leve

关 键 词：煎法 有效组分 糖尿病模型 糖脂代谢 

分 类 号：R283[医药卫生—中药学]