新疆紫草中紫草素的提取方法研究  

Studies of the Optimum Extraction Process of Alkannin from Arnebia Euchroma(Royel) Johnst in Xinjiang

作　　者：周化更 刘晓华[1] 富志军[1] 

机构地区：[1]中国药科大学中药制剂教研室,南京211198

出　　处：《海峡药学》2015年第7期30-32,共3页Strait Pharmaceutical Journal

摘　　要：目的正交法优选新疆紫草中紫草素的提取方法。方法以紫草素的含量为指标,用HPLC法测定紫草素的含量,色谱柱：Hedera ODS-2色谱柱（4.6mm×250mm,5μm）;流动相：甲醇：0.05%甲酸-水（80：20）;流速：1m L·min-1;检测波长：516nm;进样量：20μL;柱温：30℃。考察提取时间、加醇倍量、温度对紫草素的提取的影响。结果紫草素线性范围是2.5~40.0μg·m L-1,r=0.9999;平均加样回收率为99.96%。紫草中紫草素的最佳提取工艺为：提取4h,10倍量95%乙醇,温度50℃。结论该工艺合理,稳定可行,可为紫草素的提取工艺提供依据。OBJECTIVE To optimize the extraction of alkannin from Amebia euchroma （Royel） Johnst by or- thogonal design. METHODS Referring to the content of alkannin, HPLC was employed for the content determina- tion with Hedera ODS-2 （4. 6mm × 250mm,5 μm） and the mobile phase of methanol-0. 05 % formic acid solution （ 80 ：20） at detection wavelength of 516nm. The column temperature was 30℃ and flow rate was 1.0mL · min-1. Factors of temperature,times amount of 95% ethanol and extraction time were studied. RESULTS Alkannin had linear in the range between 2. 5 ~ 40. 0μg · mL-1, r = 0. 9999 ;The recovery was 99.96%. The optimal process of extraction was as follows：the extraction time was 4h, adding 10 times 95% ethanol, at 50℃. CONCLUSION The extract process is reasonable and practicable, which can be used as basis for the extraction process of alkannin.

关 键 词：紫草素 正交设计 提取工艺 HPLC 

分 类 号：R282[医药卫生—中药学]