高渗盐羟乙基淀粉溶液伍用己酮可可碱对重度失血性休克大鼠早期肠组织氧化应激损伤的影响  

Effect of hypertonic saline hydroxyethyl starch solution combined with pentoxifylline on oxidative stress injury in intestine tissue occurred in the resuscitation of severely hemorrhagic shock in rats

作　　者：耿智隆[1] 赵峰[1] 曹虹[1] 陆化梅[1] 杨木强[1] 

机构地区：[1]兰州军区兰州总医院麻醉科,甘肃兰州730050

出　　处：《西北国防医学杂志》2015年第7期424-428,共5页Medical Journal of National Defending Forces in Northwest China

基　　金：全军"十一五"基金课题资助项目(06 MA083)

摘　　要：目的:观察HSH联合PTX对重度失血性休克大鼠复苏后肠组织氧化应激的影响。方法:选择健康成年雄性SD大鼠60只,随机分为对照组(C组)、单纯休克组(NR组)、乳酸钠林格液复苏组(LR组)、高渗盐羟乙基淀粉复苏组(HSH组)、高渗盐羟乙基淀粉联合己酮可可碱复苏组(HSH/PTX组),每组12只。采用改良wigger’s法复制失血性休克模型。检测血浆中i-FABP含量及肠组织MPO、MDA、SOD含量及活性,观察肠组织病理变化。结果:与NR、LR、HSH组比较,HSH/PTX组小肠组织的病理损害明显减轻(P<0.01);MPO、MDA表达明显下降,SOD活性显著升高(P<0.01);血浆i-FABP含量明显下降(P<0.01)。结论:HSH联合PTX能抑制重度失血性休克大鼠早期复苏肠组织的氧化应激,起到肠保护作用。Objective：To observe the effect of hypertonic saline hydroxyethyl starch solution（HSH）combined with pentoxifylline（PTX）on oxidative stress injury in intestine tissue occurred in the resuscitation of severely hemorrhagic shock in rats.Methods：Sixty healthy adult male SD rats were randomly divided into control group（group C）,shock group（group NR）,sodium lactate ringer＇s solution group（group LR）,group HSH and group HSH/PTX.The hemorrhagic shock model of rat was made by improved wigger＇s method.Content of i-FABP in plasma,contents and activities of MPO,MDA,SOD in intestine tissue were detected.Pathological change was observed.Results：Compared with NR,LR and HSH groups,pathological injury and expressions of MPO and MDA in intestine tissue,and content of i-FABP in plasma were decreased while activity of SOD in intestine tissue was increased in HSH/PTX group（P〈0.01）.Conclusion：HSH/PTX solution show protective effect on intestine by suppressing the oxidative stress injury in intestine tissue occurred in the resuscitation of severely hemorrhagic shock in rats.

关 键 词：失血性休克 高渗盐羟乙基淀粉 己酮可可碱 大鼠 小肠组织 氧化应激损伤 

分 类 号：R459.7[医药卫生—急诊医学]