人成骨肉瘤MG63紫杉醇耐药细胞系建立及生物学特性研究  

Study on establishment of paclitaxel resistant osteosarcoma cell line MG63/Taxol and its biological characteristics

作　　者：吴文欣[1] 李维[2] 

机构地区：[1]湖南省第二人民医院,长沙410000 [2]中南大学湘雅三医院,湖南长沙410000

出　　处：《现代医药卫生》2015年第15期2259-2261,共3页Journal of Modern Medicine & Health

基　　金：湖南省自然科学基金资助项目(11JJ3105);湖南省科技计划项目(2013FJ3039)

摘　　要：目的建立紫杉醇(Taxol)诱导的人成骨肉瘤MG63耐药细胞株亚系MG63/Taxol,并观察其生物学特性。方法以Taxol为诱导剂,采用大剂量冲击与小剂量维持相结合的方法诱导MG63细胞,建立耐药细胞株亚系MG63/Taxol;集落形成实验检测药物敏感性;光镜观察细胞形态变化,绘制细胞生长曲线;流式细胞术检测细胞周期。结果 (1)经过6个月的诱导,建立了MG63/Taxol细胞株亚系。(2)MG63/Taxol对Taxol的耐药指数为9.50,对顺铂、甲氨蝶呤、5-氟尿嘧啶(5-Fu)也产生不同程度交叉耐药。(3)光镜观察可见MG63细胞排列规则,形态呈梭形,大小一致;MG63/Taxol细胞株亚系排列不规则,大小不均,形态呈三角形、多角形及多核现象。(4)细胞生长曲线显示MG63/Taxol细胞较亲本细胞生长缓慢。(5)细胞周期分析显示,MG63/Taxol细胞G0/G1期细胞分布增多,S期细胞分布减少。结论 (1)采用大剂量冲击与小剂量维持相结合的方法诱导建立的人成骨肉瘤多药耐药细胞株亚系MG63/Taxol细胞对顺铂、甲氨蝶呤、5-Fu产生不同程度交叉耐药。(2)MG63/Taxol细胞株亚系细胞形态、细胞生长曲线、细胞周期分布与母系细胞MG63有显著差异。Objective To establish the paclitaxel（Taxol0 induced osteosarcoma MG63 drug resistance cell subline（MG63/Taxol）and to observe its biological characteristics. Methods Paclitaxel resistant human osteosarcoma cell subline（MG63/Taxol） was established by adopting large dose impact combined with small dose maintenance to induce MG63 cell line with Taxol as the inducer. The sensitivity of chemotherapy drugs for the MG63 and MG63/Taxol cell sublines were measured by the colony for-mation assay. The cell morphology change was observed through light microscopy. The proliferation ability of cell lines was mea-sured by growth curve. The cell stages were analyzed by flow cytometry. Results（1）Through 6-month induction,paclitaxel resistant osteosarcoma cell subline（MG63/Taxol）was established.（2）The resistant index of MG63/Taxol to Taxol was 10.55. MG63/Taxol also produced the various degrees of cross resistance to cisplatin, methotrexate and 5-Fu.（3）The cell morphology was observed through light microscopy. The MG63/Taxol cells were regular arranging, spindle shape and size consistent,MG63/Taxol cell subline was irregular arranging,size inconsistent and morphology showed triangle shape,multiangular shape and multi-nucleation.（4）The cell growth cure showed that MG63/Taxol cells grew slowly than the parent cells.（5）The cell cyclical analysis displayed that the distribution of the G0/G1 stage cells was increased,while the S stage cells were decreased. Conclusion（1）A paclitaxel resistant human osteosarcoma cell subline（MG63/Taxol） established by adopting the large dose impact combined with small dose maintenance（MG63）generates the cross resistance to cisplatin,methotrexate and 5-Fu in different degrees.（2）MG63/Taxol cell subline is significantly different from the parent cell MG63 in the aspects of cell morphology,cell growth curve and cell cycle distribution.

关 键 词：骨肉瘤 细胞系 细胞凋亡 细胞增殖 药物耐受性 紫杉醇 耐药细胞 

分 类 号：R979.1[医药卫生—药品]