胃癌组织中Bmi-1表达与胃癌分化、转移及临床预后的关系  

Relationship between Bmi-1 expression and differentiation, metastasis, and clinical prognosis of gastric cancer

作　　者：何胜[1] 曾维政[1] 

机构地区：[1]中国人民解放军成都军区总医院消化内科,610083

出　　处：《现代消化及介入诊疗》2015年第3期189-192,共4页Modern Interventional Diagnosis and Treatment in Gastroenterology

摘　　要：目的研究胃癌组织中Bmi-1(B cell-specific MLV integration site-1)基因表达与胃癌分化、转移及临床预后的关系。方法研究对象为72例胃癌根治术患者的术后标本、术后病理蜡块及其完整临床资料。采用半定量逆转录聚合酶链反应(RT-PCR)技术测定胃癌组织及癌旁组织Bmi-1基因m RNA的表达,采用免疫组化对石蜡切片染色检测胃癌组织及癌旁组织Bmi-1蛋白表达水平。分析胃癌组织Bmi-1 m RNA表达与胃癌临床及病理因素之间的关系,对比m RNA表达阳性和阴性两组患者的术后1年复发率和死亡率。结果胃癌组织中的Bmi-1基因m RNA表达显著高于癌旁组织。Bmi-1基因m RNA在胃癌组织中呈强表达,在癌旁组织中呈弱表达。胃癌组织中的Bmi-1蛋白阳性表达率显著高于癌旁组织,Ⅲ-Ⅳ期胃癌组织的Bmi-1蛋白阳性表达率显著高于Ⅰ-Ⅱ期胃癌组织(P<0.05)。低分化、Borrmann分型Ⅲ-Ⅳ型、临床分期Ⅲ-Ⅳ期、浸润深度T3-T4、淋巴结转移阳性的胃癌组织Bmi-1 m RNA表达阳性率显著升高(P<0.05)。胃癌组织Bmi-1 m RNA表达阳性患者术后1年复发率显著高于阴性患者(P<0.05)。结论癌组织中Bmi-1对于胃癌分化、转移及临床预后具有一定的评价和预测作用。Objective To analyze the relationship between Bmi-1 expression and differentiation, metas- tasis and clinical prognosis of gastric cancer. Methods Postoperative specimens, pathological paraffin blocks, and complete clinical data of 72 patients who underwent surgery due to gastric cancer were selected. Semi- quantitative reverse transcription polymerase chain reaction （RT-PCR） was used to determine mRNA expres- sion of Bmi-1 gene in gastric cancer and adjacent tissues. Irnmunohistochemical staining of paraffin section was performed to detect Bmi-1 protein levels in gastric cancer and adjacent tissues. Relationship between Bmi-1 mRNA expression and various clinical and pathologic factors was explored. The 1-year recurrence rate and mortality was compared between patients with Bmi-1 mRNA positive and negative. Results Bmi-1 mR- NA expression in gastric cancer was significantly higher than that in adjacent tissues. Bmi-1 mRNA expression was strongly expressed in gastric cancer and weakly expressed in adjacent tissues. Bmi-1 protein expression was significantly higher in gastric cancer than that in adjacent tissues. Bmi-1 protein expression was signifi- cantly higher than in Ⅲ-IV gastric cancer than that in I - Ⅱ gastric cancer （P 〈 0.05）. Bmi-1 mRNA expres- sion was significantly higher in patients with poorly differentiation, Bomnann type HI-IV, clinical stage Ⅲ- IV, depth of invasion （T3-T4）, and lymph node metastasis （P 〈0.05）. The 1-year recurrence rate in Bmi-1 mRNA-positive patients was significantly higher than negative patients （P 〈0.05）. Condasion The Bmi-1 has a certain evaluation and prediction value for the differentiation, metastasis, and clinical prognosis of gastric cancer.

关 键 词：胃癌 BMI-1 半定量逆转录聚合酶链反应 免疫组化 

分 类 号：R331.2[医药卫生—人体生理学]