针叶樱桃提取物片剂对小鼠增强免疫力的研究  

Effects of Tablets of Acerola Cherry Extract on Immunity of Mice

作　　者：田玉琼[1] 蒋中仁[1] 田嘉军[1] 邹思颖[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041

出　　处：《预防医学情报杂志》2015年第7期535-540,共6页Journal of Preventive Medicine Information

摘　　要：目的探讨针叶樱桃提取物片剂对小鼠增强免疫力的研究。方法昆明种小鼠160只,全雌,体重18∽22 g。随机分为4组,每组40只动物,其中一组用于小鼠腹腔巨噬细胞吞噬鸡红细胞实验;2组用于NK细胞活性测定及淋巴细胞转化实验;3组用于抗体生成细胞实验、血清溶血素测定和迟发性变态反应实验;4组用于小鼠碳廓清实验。每1组又分为蒸馏水空白对照组（阴性对照组）、低剂量组、中剂量组、高剂量4个剂量组。受试物设333 mg/kg.BW（低剂量）、667mg/kg.BW（中剂量）、1 000 mg/kg.BW（高剂量）3个剂量组。小鼠按20 ml/kg.BW体重1次经口灌胃,至少连续给予30d,每周称1次体重,调整灌胃体积。试验结束时称体重后处死动物,取脾脏和胸腺在电子分析天平上称重,并计算脏/体比值。结果针叶樱桃提取物片剂连续灌胃小鼠30 d后,对小鼠的体重、脏器/体重比值无明显影响,对小鼠的细胞免疫试验结果阴性,对小鼠的单核—巨噬细胞吞噬功能试验结果阴性;对小鼠的体液免疫试验结果阳性,对小鼠NK细胞活性试验结果阳性。结论表明针叶樱桃提取物片剂对昆明种小鼠有增强免疫力的作用。Objective To study the effects of tablets of acerola cherry extract on immunity of mice. Methods Altogether 160 female Kunming mice with body weight 18- 22 g were randomly divided into 4 groups,40 mice in each group. The first group was applied for phagocytosis test by peritoneal macrophages with chicken erythrocyte,the second group for determination of the activity of NK cells and lymphocyte transformation,the third for antibody forming cell experiment,serum hemolysin assay,delayed allergic reaction experiment and the fourth for mice carbon clearance test. In each group three doses including 333 mg / kg. BW,667 mg / kg. BW,1 000 mg / kg. BW and one negative control were set. The mice were administered with oral gavage at least 30 days,with a dose of 20 ml / kg. BW. At the end of the experiment,the organ / body weight ratio of spleen and thymus were evaluated. Results Tablets of acerola cherry extract showed no significant effects on the weight and organ / body weight ratio. The phagocytic function of monocyte macrophage and cell immunity test exhibited negative,wihle humoral immunity and NK cell activity test were positive. Conclusion Tablets of acerola cherry extract has enhancing effect on immunity of mice.

关 键 词：针叶樱桃提取物片剂 小鼠 增强免疫力 

分 类 号：R33[医药卫生—人体生理学]