雷帕霉素对人脐带间充质干细胞衰老抑制的作用研究  

作　　者：王巧玲[1] 徐辰[1] 王越[1] 刘厚奇[1] 

机构地区：[1]第二军医大学基础部组织学与胚胎学教研室,200433

出　　处：《浙江临床医学》2015年第8期1316-1318,共3页Zhejiang Clinical Medical Journal

摘　　要：目的：通过探讨雷帕霉素对人脐带来源间充质干细胞（UC-MSCs）衰老的影响，推动其临床应用。方法将人脐带间充质干细胞（MSCS）分为对照组及雷帕霉素组。β-半乳糖苷酶染色实验检测UC-MSC细胞内衰老相关β-半乳糖苷酶（SABG）的表达量；细胞计数试剂（CCK8）检测UC-MSC细胞的增殖；Real-time PCR检测UC-MSC细胞中衰老、增殖与干性相关 mRNA的表达。结果与无水乙醇对照组相比，雷帕霉素组细胞β-半乳糖苷酶染色阳性率及染色深度均降低；细胞增殖加快；增殖、干性基因升高同时伴有衰老相关基因的下降。结论雷帕霉素能够减缓人脐带MSCs的衰老。Objective To investigate the effect of Rapamycin in the senescence process of umbilical cord-derived mesenchymal stem cells （UC-MSCs）.Methods UC-MSCs were divided into Rapamycin group and absolute alcohol control group.The measure of senescence-associated b-galactosidase（SABG） of UC-MSCs were detected by Beta galactosidase staining reagent.The proliferation performance of UC-MSC were examined by Cell Counting Kit-8（CCK8）.The expressions of senescence,proliferation and stemness relative mRNA were examined by real-time PCR. Results Compared with the absolute alcohol control group,The Rapamycin group show a lower positive rate and dyeing depth of Beta galactosidase staining. Rapamycin treated UC-MSCs present a higher rate of proliferation.The expressions of proliferation,stemness related mRNA were elevated, with an decrease of senescence relative mRNA.Conclusion Rapamycin slows down the senescence phenotype.

关 键 词：雷帕霉素 间充质干细胞 增殖 衰老 

分 类 号：R722.12[医药卫生—儿科]