荷斯坦母牛生长激素受体基因F279Y突变与产奶性状的关联分析  

Association between F279Y mutation of growth hormone receptor gene and milk production traits in Holstein cows

作　　者：郭晓飞[1] 徐小兵[2] 李秋玲[3] 冯涛[2] 狄冉[1] 刘秋月[1] 胡文萍[1] 王翔宇[1] 安永福[4] 储明星[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与种质创新重点实验室,北京100193 [2]北京市农林科学院畜牧兽医研究所,北京100097 [3]廊坊师范学院生命科学学院,廊坊065000 [4]河北省畜牧兽医研究所,保定071000

出　　处：《安徽农业大学学报》2015年第4期515-519,共5页Journal of Anhui Agricultural University

基　　金：中国农业科学院科技创新工程(ASTIP-IAS13);国家科技支撑计划(2006BAD04A10);廊坊师范学院博士基金(LSLB2014048)共同资助

摘　　要：采用PIRA-PCR和RFLP法检测204头中国荷斯坦母牛生长激素受体(growth hormone receptor,GHR)基因F279Y突变,并分析其与产奶性状的关系。结果在中国荷斯坦母牛中检测到GHR基因F279Y突变,3种基因型TT、TA和AA的频率分别为0.461、0.495和0.044,等位基因T和A的频率分别为0.708和0.292。分析表明,AA基因型个体305天产奶量显著高于TA和TT基因型(P<0.05);TT基因型个体乳脂率显著高于AA基因型个体(P<0.05),乳蛋白率极显著高于TA和AA基因型个体(P<0.01)。A等位基因对305天产奶量有正加性效应,显性度相对较高;而对其他4个乳成分性状有负加性效应,显性度相对较低。等位基因A具有提高产奶量的效应,等位基因T具有提高乳成分的效应。本研究初步表明F279Y突变可以用于中国荷斯坦牛产奶性状的标记辅助选择。The F279Y mutation of the GHR gene was detected in 204 Chinese Holstein cows using primer-introduced restriction analysis-polymerase chain reaction （PIRA-PCR） and restriction fragment length polymorphism （RFLP）. The F279Y mutation and three genotypes were identified in Chinese Holstein cows. Genotype frequency of TT, TA and AA was 0.461, 0.495 and 0.044, respectively. Allele frequency of T and A was 0.708 and 0.292, respectively. The relationship between the F279Y mutation and milk production traits in Holstein cows was analyzed using linear least squares model. Least squares mean of 305-day milk yield in individuals with AA genotype was significantly higher than that with TA （P〈0.05） or TT （P〈0.05）. Least squares mean of fat percentage in individuals with TT genotype was significantly higher than that with AA （P〈0.05）. Least squares mean of protein percentage in individuals with TT genotype was significantly higher than that with TA （P〈0.01） or AA （P〈0.01）. Allele A had a positive additive effect on 305-day milk yield with a higher dominance and had a negative additive effect on other four milk composition traits with a lower dominance. A is the dominant allele for milk yield, while T is the dominant allele for milk composition. These results preliminarily indicated that the F279Y mutation of the GHR gene could be used in molecular marker-assisted selection programs of milk production traits in Holstein cows.

关 键 词：荷斯坦母牛 生长激素受体基因 F279Y突变 产奶性状 

分 类 号：S823.2[农业科学—畜牧学]