复方中药抑制大鼠肥大细胞脱颗粒的作用及其机制  

Inhibitory Effect of Chinese Herbal Compound on Mast Cell Degranulation in Rats and Its Mechanism of Action

作　　者：何伟逸[1] 杨成彬[1] 刘晓宇[1] 黄海珍[1] 杨平常[1] 刘志刚[1] 

机构地区：[1]深圳大学过敏反应与免疫学研究所,广东深圳518060

出　　处：《南昌大学学报（医学版）》2015年第3期1-5,9,共6页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(31101280;81271950;81373176);广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009);广东省自然科学基金(S2012010008514);深圳市重点实验室组建项目(SW201110010);深圳市科技计划国际科技合作项目(GJHZ20130408174112021)

摘　　要：目的探讨复方中药(Formula-3)抑制大鼠肥大细胞脱颗粒的作用及其机制。方法通过DNP-BSA-IgE激发建立致敏的RBL-2H3细胞模型。采用MTT实验检测Formula-3(中药组成:人参9g,干姜9g,黄连9g,当归9g,乌梅30g,灵芝30g)对细胞活性的影响;利用酶联免疫吸附法研究Formula-3对致敏肥大细胞β-氨基己糖苷酶及细胞因子的影响;共聚焦激光扫描荧光显微镜观察检测SOC通道的变化。结果成功建立致敏的细胞模型。Formula-3不会对细胞活性造成影响。Formula-3可减少致敏肥大细胞β-氨基己糖苷酶的释放,Th2型细胞因子和Th1型细胞因子的水平亦有显著变化(P<0.05);能够抑制肥大细胞膜上SOC通道的开放,Ca2+瞬变从1.83降至1.18(P<0.05)。结论 Formula-3通过抑制SOC通道的开放达到抑制肥大细胞脱颗粒的目的。Objective To investigate the inhibitory effect of Chinese herbal compound （Formu-la-3）on mast cell degranulation in rats and its mechanism of action.Methods RBL-2H3 cells were sensitized with DNP-BSA-IgE.The effect of Formula-3 （ginseng 9 g,dried ginger 9 g,Coptis chinensis 9 g,angelica 9 g,dark plum 30 g and lucid ganoderma 30 g）on cell viability was evalua-ted by MTT analysis.The effect of Formula-3 onβ-hexosaminidase and cytokines was evaluated by ELISA.The change in SOC channel was observed using confocal laser scanning microscopy. Results The sensitized cell model was established successfully.Formula-3 had no effect on cell viability.However,it inhibitedβ-hexosaminidase release and changed the levels of Th2-type cyto-kines and Th1-type cytokines （P 〈0.05）.Furthermore,Formula-3 inhibited SOC channel opening and reduced Ca2＋ transients from 1.83 to 1.18 （P 〈 0.05）.Conclusion Formula-3 can inhibit mast cell degranulation through inhibiting SOC channel opening.

关 键 词：复方中药 Ⅰ型变态反应 肥大细胞 脱颗粒 细胞因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]