环丙沙星体外诱导肺炎克雷伯菌耐药机制的研究  

Mechanisms of Ciprofloxacin-Induced Resistance in Klebsiella Pneumoniae in Vitro

作　　者：韩菊梅[1] 叶晓光[2] 

机构地区：[1]广东工业大学医院内科,广州510006 [2]广州医科大学第二附属医院感染科,广州510260

出　　处：《南昌大学学报（医学版）》2015年第3期37-39,共3页Journal of Nanchang University：Medical Sciences

摘　　要：目的探讨环丙沙星体外诱导敏感肺炎克雷伯菌(KPn)耐药的分子机制。方法对临床分离的敏感肺炎克雷伯菌体外使用环丙沙星,采用多步法诱导耐药,并随机选择10对临床分离敏感菌和诱导后高度耐药菌进行GyrA基因QRDR(喹诺酮抗性区,quinolone resistant-determining region)的聚合酶链式反应(PCR)扩增、SDSPAGE电泳鉴定外膜外排泵蛋白TolC及膜孔蛋白porin蛋白的表达。结果诱导后的KPn均成为耐药菌,其中高度耐药菌占73.81%。10株诱导的耐药菌中Ser83突变率为30%;膜孔蛋白porin表达均减少,外排泵蛋白TolC表达均增多。结论环丙沙星的使用与KPn耐药密切相关,并可引起KPn多种耐药分子产生。Objective To investigate the molecular mechanisms of ciprofloxacin-induced resist-ance in Klebsiella pneumonia （KPn）in vitro.Methods The antibiotic resistance of Klebsiella pneumoniae isolated from patients was induced by ciprofloxacin in vitro using multi-step test method.Ten pairs of susceptible clinical isolates and ciprofloxacin-induced resistant strains were randomly selected in this study.The quinolone resistance determining region （QRDR）of gyrA gene was amplified by PCR,and the expression of TolC protein and porin protein were detected by SDS-PAGE.Results All KPn showed drug resistance after ciprofloxacin induction,and highly drug-resistant bacteria accounted for 73.81%.Among the 10 ciprofloxacin-induced resistant strains,the mutation rate of Ser83 was 30%.In addition,porin expression decreased but TolC ex-pression increased after ciprofloxacin induction.Conclusion The use of ciprofloxacin is closely related to drug resistance and causes the production of a variety of drug-resistant molecules in KPn.

关 键 词：肺炎克雷伯菌 环丙沙星 体外诱导 GyrA 基因 TOLC 蛋白 膜孔蛋白 PORIN 

分 类 号：R978.1[医药卫生—药品]