换液培养对猪卵母细胞的成熟及胚胎体外发育的影响  

Effects of Culture Medium on Porcine Oocyte Maturation and Embryo Development in Vitro

作　　者：罗绿花[1] 石俊松[1] 周荣[1] 麦然标[1] 纪红美 余婉娴[1] 蔡更元[2] 王青来[1] 吴珍芳[2] 

机构地区：[1]广东温氏食品集团股份有限公司国家生猪种业工程技术研究中心,广东云浮527439 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《广东畜牧兽医科技》2015年第4期27-30,共4页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省家畜分子与细胞工程育种技术研究团队(2011A020102003);创建肉鸡;肉猪种业国家工程技术研究中心能力建设(2012B07071006)

摘　　要：通过后期去除激素培养比较了卵丘卵母细胞成熟及构建克隆胚后的发育情况,同时孤雌胚胎和体外受精胚胎体外培养48 h后全量换液,比较了其卵裂率和囊胚率之间的差异。结果显示,卵丘卵母细胞培养22h后换成不含激素的成熟培养液继续培养至44 h,其成熟率与对照组无显著差异(P>0.05);后期克隆胚胎卵裂率和囊胚率与对照组均无显著差异(P>0.05)。孤雌胚胎换液培养卵裂率高于未换液组,囊胚率低于未换液组,但差异均不显著(P>0.05)。体外受精胚胎换液培养卵裂率以及囊胚率和未换液组差异不显著(P>0.05)。本实验结果说明换液培养对卵母细胞的成熟及后续的体细胞克隆胚发育无显著影响,孤雌和体外受精胚胎换液培养对后期发育也无显著影响。This study examined the effects ofthe late removal ofthe hormone to the oocytes mature and the cloned embryos development, and parthenogenetic embryos and IVF embryos in vitro after 48 h full amount in the culture medium, compares the difference between the cleavage rate and blastocyst rate.Results showed that the Cumulus Cells continued to develop to 44 hours after changing to no hormone culture medium at 22 hours regular culture, the rate of maturity with no significant difference to the control group （P 〉0.05）; the late cloned embryo cleavage rate and blastocyst rate and the control group had no significant difference （P 〉 0.05）. After changing the culture mudium, parthenogenetic embryos cleavage rate is higher than regular culture medium group, blastocyst rate is lower than normal culture medium group, with no significant difference （P 〉 0.05）.To IVF embryos,culture medium changing were no significant difference （P 〉 0.05）.The experiment results show that changing the culture medium had no significant effect on oocytes maturation and subsequent somatic cell cloning embryos, parthenogenetic and IVF embryos.

关 键 词：猪 卵母细胞 胚胎发育 

分 类 号：S814.6[农业科学—畜牧学]