刚果红平板法筛选血链球菌胞外多糖缺失突变株  

Screening of Streptococcus sanguis Exopolysaccharide Mutant by Congo Red Agar Plate

作　　者：郝苗[1] 金莺[1] 李婷[1] 尚萌萌[1] 郑兰艳[1] 

机构地区：[1]中国医科大学基础医学院病原生物教研室,辽宁沈阳110122

出　　处：《微生物学杂志》2015年第3期54-57,共4页Journal of Microbiology

基　　金：国家自然科学基金项目(81170211)

摘　　要：细菌胞外多糖不但能够保护细菌,防止外界环境变化和侵蚀者对菌体细胞的侵害,而且在医学微生物学中还具有特有的生物学活性,包括抗辐射、抗氧化等特性。尽管细菌胞外多糖受到了学者的广泛关注,但胞外多糖的生物合成机制还不十分清楚。以血链球菌为模式生物,以随机插入突变机制构建的血链球菌胞外多糖形成基因文库为受试对象,建立了一种简单易行的刚果红平板法用于筛选血链球菌细胞外粘多糖形成缺失突变株的方法,为日后研究细菌胞外多糖形成机制以及临床上筛选胞外多糖缺失突变株奠定了基础。Exopolysaccharide （EPS） of bacteria not only can protect the cell from the changes of adverse circumstances and invaders, but also has special biological activities in medical microbiology, including anti-radiation, anti-oxidation, and other properties. Although the EPS has caught the researchers＇ extensive attention, yet the synthesis mechanism of EPS is still unclear. In this study, Streptococcus sanguis was used as a model organism, and a gene li- brary of the EPS formation mechanism by S. sanguis built by random insertion mutagenesis technique as tested subject, an easy and practicable Congo red agar plate method was developed for screening the EPS deficient mntants, in order to provide a foundation for studying the formation mechanism of the EPS and screening the S. sanguis exopolysaccharide mutants in clinic in the days to come.

关 键 词：胞外多糖(EPS) 刚果红平板 血链球菌 

分 类 号：Q93-33[生物学—微生物学] R378.12[医药卫生—病原生物学]