荜茇宁衍生物对高脂血症大鼠调脂作用及其机制的研究  

The function of lipid regulation and mechanism study of piperlonguminine derivative(GBNT) in rats with hyperlipidemia

作　　者：阿荣[1,2] 乔延江[3] 博.格日勒图[4] 昭日格图[4] 包力尔[4] 

机构地区：[1]内蒙古农业大学理学院,内蒙古呼和浩特010020 [2]北京中医药大学中药学院,北京100102 [3]北京中医药大学中药信息工程研究中心,北京100102 [4]内蒙古大学蒙药化学研究所,内蒙古呼和浩特010021

出　　处：《安徽医药》2015年第8期1446-1449,共4页Anhui Medical and Pharmaceutical Journal

基　　金：内蒙古自治区自然科学基金项目(No 21400-5143307)

摘　　要：目的探讨荜茇宁(GBN)的衍生物(GBNT)对高脂血症大鼠的调脂作用,并考察其作用机制。方法高脂饲料喂养Wister大鼠,建立高脂血症模型,造模同时连续灌胃给药8周后,腹主动脉取血,检测各组大鼠血清中总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C)含量;HE染色检测肝脏病理学形态;Western blot方法研究低密度脂蛋白受体(LDLR),卵磷脂胆固醇脂酰基转移酶(LCAT),胆固醇7α-羟化酶(CYP7A)蛋白表达的变化。结果GBNT低、高剂量组均能降低模型大鼠TC、TG和LDL-C的含量,升高HDL-C含量;上调LDLR,LCAT,CYP7A1蛋白的表达;并减轻肝脏脂肪变性。结论 GBNT具有降低高脂血症模型大鼠血脂水平的作用,其机制可能与调节LCAT,CYP7A1等脂质代谢酶表达有关。Objective To explore lipid regulation and mechanism of piperlonguminine derivative（GBNT）in rats with hyperlipidemia. Methods High fat diet was fed for hyperlipidemia model to Wister male rats.Meanwhile,piperlonguminine （GBN）and GBNT were intragastric administrated for 28 d respectively.Blood was isolated from abdominal aorta.The concentration of total cholesterol （TC）, triglyceride （TG）,low density lipoprotein cholesterol （LDL-C）,and high density lipoprotein cholesterol （HDL-C）in serum were meas-ured.HE staining method assayed the pathology morphology of liver.Finally,the levels of low density lipoprotein cholesterol receptor （LDLR）,lecithin cholesterol acyltransferase （LCAT）,and cholesterol 7 alpha hydroxylase （CYP7A1）were assayed by western blot. Results The low and high dose of GBNT could reduce levels of TC,TG,and LDL-C,increase levels of HDL-C,up -regulate the ex-pression of LDLR,LCAT and CYP7A1,and relieve liver fatty degeneration.Conclusions GBNT could reduce serum lipid levels in rats with hyperlipidemia,which might be related to regulating lipid metabolic enzyme including LCAT and CYP7A1.

关 键 词：荜茇宁衍生物(GBNT) 高血脂 脂质代谢酶 

分 类 号：R285.5[医药卫生—中药学]