卷柏多糖抗肠道病毒71型活性及机制研究  

Anti-Enterovirus 71 and Mechanism of Polysaccharides Extracted from Selaginella tamariscina

作　　者：夏燕平[1,2] 马腾[3] 齐向云[2] 李庆军[1,2] 王升启[1,2] 杨静[1,2] 

机构地区：[1]河南中医学院药学院,郑州河南450008 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]航天中心医院心脏病诊疗科,北京100049

出　　处：《生物技术通讯》2015年第4期454-459,共6页Letters in Biotechnology

基　　金：国家自然科学基金(31270197;81473184);国家高技术研究发展计划(2012AA022501);国家科技重大专项(2013ZX09304102;2012ZX09301003-001-004)

摘　　要：目的:研究卷柏多糖对肠道病毒71型(EV71)的抑制作用,并初步探讨其抗病毒机制。方法:测定30%、50%醇沉卷柏多糖对Vero细胞的毒性和对EV71感染致Vero细胞病变的抑制作用,筛选出选择指数(SI)高的卷柏多糖醇沉部位,检测活性高的卷柏多糖部位对EV71的直接灭活作用及对病毒生活周期的影响。结果:30%和50%醇沉卷柏多糖及利巴韦林抑制EV71致细胞病变的IC50值分别为23.62±2.50、10.43±0.38和38.66±4.91 mg/L,SI分别为>169、>384和101,提示50%醇沉卷柏多糖抗EV71活性最好。机制研究显示,50%醇沉卷柏多糖对EV71无直接杀病毒作用;与模型组相比,50%醇沉卷柏多糖可显著抑制EV71对Vero细胞的吸附作用(P<0.05),而对EV71的进入、复制及释放环节无影响。结论:卷柏多糖具有抗EV71活性,可能通过抑制病毒的吸附达到抗病毒作用。Objective：To study the inhibitory activity and mechanism of polysaccharides extracted from Selaginella tamariscina using Vero cells infected with enterovirus 71（EV71）.Methods：Cytotoxicity and cytopathic effect（CPE） inhibition of 30%,50% alcohol precipitated polysaccharides from S.tamariscina were measured and theagent with the higher selection index（SI） was determined.Then it was added to Vero cells infected with EV71 tostudy its direct virucidal activity,and acting phase by time-of-addition assays.Results：IC50 of 30%,50% alcoholprecipitated polysaccharides and ribavirin were 23.62±2.50,10.43±0.38 and 38.66±4.91 mg/L,respectively,and SIof the agents were respectively 〉169,〉384 and 101.50% alcohol precipitated polysaccharides showed the best an-ti-EV71 activity.50% alcohol precipitated polysaccharides had no direct virucidal effect on EV71,but it could in-hibit the attachment of EV71 with Vero cells significantly（P〈0.05）,but not viral penetration,replication and re-lease phase.Conclusion：50% alcohol precipitated polysaccharides from S.tamariscina has remarkable anti-EV71 activity by blocking virus attachment.

关 键 词：多糖 卷柏 肠道病毒71型 VERO细胞 病毒吸附 

分 类 号：R25[医药卫生—中医内科学] Q75[医药卫生—中医学]