安替可胶囊的质量标准研究  

Study on the Quality Standard of Antike Capsule

作　　者：缪珊[1] 韩超[2] 毕琳琳[1] 李捷[1] 张雅[1] 王四旺[1] 石小鹏[3] 

机构地区：[1]第四军医大学药学系,陕西西安710032 [2]北京军区总医院,北京100049 [3]第四军医大学西京医院,陕西西安710032

出　　处：《中医药导报》2015年第16期40-42,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省社会发展攻关计划(2012K14-05-04)

摘　　要：目的：建立安替可胶囊的质量标准。方法：采用薄层色谱法对方中的当归、蟾皮进行定性鉴别；采用HPLC法对制剂中华蟾酥毒基和脂蟾毒配基进行定量分析。结果：薄层色谱鉴别分离效果好，专属性强；华蟾酥毒基在50-250mg·L-1范围内线性关系良好（r=0．9999）；脂蟾毒配基在44-220mg·L-1范围内线性关系良好（r=0．9999）；华蟾酥毒基平均加样回收率为99．79％，RSD％=0．56％；脂蟾毒配基平均加样回收率为100．08％，RSD％=0．47％。结论：该方法简单、准确、专属性强、重复性好，可有效控制安替可胶囊的质量。Objective： To establish the quality standard of Antike capsule. Method： Danggui （Radix Angelicae Sinensis） and Chanpi （toad skin） in Antike capsule were identified by TLC. The content of cinobufagin and bufogenin were determined by HPLC. Results： The characteristic identification by TLC was distinct and specific. The Linear ranges of cinobufagin and bufo- genin were 50-250 mg·L-1 and 44-220 mg·L-1 with good correlation coefficient （r=0.9999）, respectively. The average recovery of cinobufagin was 99.79% and RSD was 0.56%. The average recovery of bufogenin was 100.08% and RSD was 0.47%. Conclusion： The established methods are accurate, exclusive, reproducible and suitable for the quality control of Antike capsule.

关 键 词：安替可胶囊 质量标准 薄层色谱 HPLC 华蟾酥毒基 脂蟾毒配基 

分 类 号：R284.1[医药卫生—中药学]