miR-135b在鼻咽癌中的表达及其意义  

The Expression of miR-135b in Nasopharyngeal Carcinoma and its Significance

作　　者：唐金凤[1] 鲁耀邦[2] 胡文铧[3] 王永存[4] 王思捷[1] 杨腾[1] 吴平[1] 

机构地区：[1]广东医学院附属医院临床医学研究中心,广东湛江524001 [2]湖南中医药大学药学院,湖南长沙410208 [3]广东医学院附属医院病理中心,广东湛江524001 [4]广东医学院附属医院肿瘤中心,广东湛江524001

出　　处：《湖南中医药大学学报》2015年第7期50-53,共4页Journal of Hunan University of Chinese Medicine

基　　金：广东医学院科研基金面上培育项目资助(M2014049)

摘　　要：目的探讨mi R-135b在鼻咽癌(NPC)中的表达情况及与临床病理特征的关系。方法收集鼻咽患者活检组织92例,其中NPC组织67例,鼻咽黏膜慢性炎症(CIONM)组织25例。通过q PCR方法检测mi R-135b在NPC组织和CIONM组织中的相对差异表达,利用SPSS软件统计分析mi R-135b与NPC临床病例特征的相关性。结果 (1)mi R-135b在NPC组织中的表达量明显高于CIONM组织(P<0.01);(2)mi R-135b在不同性别中亦存在明显差异,92例鼻咽患者和67例NPC患者中均存在男性的表达明显高于女性(P<0.01);(3)mi R-135b的表达与年龄有相关性(r=0.213,P<0.05);(4)mi R-135b在淋巴结转移中起重要作用,在淋巴结转移患者中mi R-135b的表达高于无淋巴结肿大的患者(P<0.05)。结论在NPC患者中mi R-135b在年龄分组、性别分组及淋巴结是否转移分组中均存在表达差异。mi R-135b是NPC的危险因素,为后续探索NPC早期诊断的分子标记物提供理论基础。Objective To investigate the expression of miR-135b and clinicopathological features of relevance in nasopharyngeal carcinoma（NPC）. Methods 92 cases of nasopharyngeal biopsies were collected, in which were 67 cases of NPC patients and 25 cases of Chronic inflammation of nasopharyngeal mucosa （CIONM） patients. The expression of miR-135b relative differences in NPC tissues and NC tissues were analyzed by qPCR methods. The correlation of miR-135b and nasopharyngeal characteristic was analyzed by using SPSS software. Results （1） The expression of miR-135b in NPC was significantly higher than CIONM （P〈0.01）. （2） The expression of miR-135b was significant differences in gender, and 92 cases of nasopharyngeal biopsies and 67 cases of NPC male patients were higher than the female patients （P〈0.01）. （3） The expression of miR-135b were correlated with age （r=0.213, P〈0.05）. （4） MiR-135b plays an important role in lymph node metastasis, the expression levels of miR-135b in patients with lymph node metastasis was significantly higher than in patients without lymph node enlargement （P〈0.05）. Conclusion The miR-135b in the age, gender and lymph node metastasis groups were differentially expressed in NPC patients. The miR-135b that is a risk factor of NPC nasopharyngeal carcinoma provides a theoretical basis for further exploration of the early diagnosis of NPC molecular markers.

关 键 词：鼻咽癌 miR-135b 淋巴结转移 分子标记物 

分 类 号：R446.8[医药卫生—诊断学]