赣南某矿区土壤中抗镉菌株的分离筛选及对镉的吸附特性  

Separating,Filtering and Cadmium Adsorption of Cadmium Resistant Microbe from Mining Area Soil in Gannan

作　　者：聂锦霞[1] 陈明[1] 蔡青云[1] 周丹[1] 

机构地区：[1]江西理工大学江西省矿冶环境污染控制重点实验室,江西赣州341000

出　　处：《有色金属工程》2015年第4期85-89,共5页Nonferrous Metals Engineering

基　　金："十二五"国家科技支撑计划项目(2012BAC11B07);江西省自然科学基金资助项目(20132BAB213017);江西理工大学科研基金资助项目(NSFJ2015-G02)

摘　　要：采用稀释平板分离技术,在培养基中加入不同浓度的Cd2+,经过分离和驯化,从赣南受污染矿区中筛选出耐镉菌株E和菌株M,E为丝状革兰氏阴性菌,M为卵圆形革兰氏阳性菌。采用革兰氏染色法对两菌株进行形态学初步鉴定,利用摇床培养方法研究菌株的生长曲线、温度和pH值等特性,利用能谱仪测定菌株对镉的生物吸附。结果表明,两菌株对不同浓度的Cd2+溶液均有一定的吸附能力,且菌株M较菌株E的吸附率更高。吸附镉后的菌株均出现明显的镉峰。By using dilution plate method,the fungus E and fungus M are filtered from the mining soil of the Gannan through the separating and taming at different concentration of Cd in cultural base. The fungus E is the filamentous gram-negative bacteria and the fungus M is the rod-shaped gram-positive bacteria. The morphology of two fungus is preliminarily identified through the Gram staining. The characters such as growing curve,optimal temperature and p H value for two fungus are investigated by the oscillator. The biosorptions for Cd with two fungus are measured by Energy Dispersive Spectrometer（ EDS）. The results show that the fungus E and fungus M are of the certain adsorption capacity for Cd2 ＋in different initial concentration,and the adsorption rate with the fungus M is higher than that with the fungus E. There are the significant Cd peaks on the fungus E and fungus M after adsorption.

关 键 词：重金属镉 抗性微生物 分离筛选 吸附性能 

分 类 号：X53[环境科学与工程—环境工程] X172