HIV感染1年内不同形式HIV DNA的动态变化情况  

HIV-1 DNA dynamics during the first year of HIV-1 infection

作　　者：陈广磊[1] 焦艳梅[2] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心,北京100069 [2]首都医科大学附属北京佑安医院放射科,北京100069

出　　处：《中国病毒病杂志》2015年第3期176-180,共5页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金(81371803);国家"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2012ZX10001-003);艾滋病研究北京市重点实验室项目(BZ0089);国家临床重点专科建设项目(WJWYA-2014-006);北京市卫生系统高层次卫生技术人才项目(焦艳梅)

摘　　要：目的探究人类免疫缺陷病毒(HIV)感染者感染1年内HIV-1总DNA、2-LTR环状DNA和整合型DNA的变化情况。方法从HIV急性期队列中选取疾病进展速度不同的两组患者,"高CD4组"CD4计数在1年内大于500个/μl,"低CD4组"CD4计数在1年内小于300个/μl。比较两组患者在同一感染时间点时总HIV DNA、2-LTR环状HIV DNA及整合HIV DNA的水平,及同一组患者在不同感染时间点时的总HIV DNA、2-LTR环状HIV DNA和整合HIV DNA的水平。结果 "高CD4组"和"低CD4组"间总HIV DNA在感染第12个月时差异有统计学意义(U=2.958,P<0.05),2-LTR环状HIV DNA及整合HIV DNA在两组间差异无统计学意义。"高CD4组"总HIV DNA在感染第1个月和第12个月间差异有统计学意义(H=2.502,P<0.05),"低CD4组"各个月份间差异无统计学意义。结论对于急性HIV感染的患者,尤其是感染1年内的患者,总HIV DNA有较好的临床应用价值,可以辅助预测患者的疾病进展情况。Objective To study the dynamic changes of total,2-LTR circular and integrated HIV-1DNA during the first year of HIV-1infection. Methods Two distinct HIV-1patient groups from the acute infection cohort with different disease progression were recruited.During the first year of HIV-1infection,the CD4 count in ＂ High CD4Group＂ remained above 500cells/μl,while the ＂ Low CD4Group＂ progressed below 300cells/μl.We compared the total,2-LTR circular,and integrated HIV-1DNA at the same infection time between the two groups,and the HIV DNA level at different infection months in the same group. Results The difference of total HIV DNA was significant different at the 12-month point between ＂ High CD4Group＂ and ＂ Low CD4Group＂（U=2.958,P〈0.05）.The total HIV DNA in ＂ High CD4Group＂ also showed significant difference between 1-month and 12-month infection points（H=2.502,P〈0.05）. Conclusions For acute HIV-1infection especially patients infected within one year,the total HIV DNA may help us to predict the disease progression.

关 键 词：HIV感染 HIV DNA CD4计数 病毒载量 

分 类 号：R512.91[医药卫生—内科学]