藜异型性种子后代植株对NaCl和KCl短期胁迫的响应  

Physiological Responses of Plants Derived from the Heteromorphic Seeds of Chenopodium album to Salt Stress

作　　者：兰欣欣 姚世响[1] 兰海燕[1] 

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2015年第6期1086-1092,共7页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(31460043;30660012);新疆维吾尔自治区优秀青年科技人才培养项目(2013721013)

摘　　要：【目的】研究不同盐(Na Cl和KCl)胁迫对藜(Chenopodium album)的抗氧化系统的影响,探讨异型种子后代植株之间对盐胁迫生理响应机制的可能差异。【方法】以藜异型性种子后代植物为材料,测量藜黑色及褐色种子在不同浓度(0、50、300 mmol/L)Na Cl或KCl短时间胁迫下相关生理指标(氧自由基、MDA;SOD、CAT、POX)。【结果】在不同浓度(0、50、300 mmol/L)Na Cl或KCl短时间胁迫下,藜黑色及褐色种子后代植株活性氧水平随时间有升高但不显著,异型性种子后代植株之间无显著差异;MDA随盐胁迫时间增加,呈显著上升趋势,Na Cl胁迫上升更明显。短时间盐胁迫下,两种种子后代中抗氧化酶活性(SOD、CAT、POX)均呈上升趋势,CAT和POX增加更显著;Na Cl比KCl胁迫,低浓度比高浓度盐,对氧化酶活性的促进更明显。以上参数在异型性种子后代植株间未显示显著差异。【结论】抗氧化酶在藜耐受短期高盐(Na Cl或KCl)胁迫过程中发挥重要作用;藜异型性种子后代植株在短期盐胁迫下表现相似的生理生化特性。【Objective】In the present study,comparison of the antioxidative enzymes responses to salinity stress between the plants from brown and black seeds of Chenopodium album was conducted.【Method】Heteromorphic seeds of Chenopodium album were taken as material,the activity of some important physiological responses（ O2.-,MDA; SOD,CAT,POX） were measured under short time treatment of different concentrations（ 0,50,300 mmol/L） of Na Cl and KCl.【Result】The results indicated that with short time treatment of different concentrations（ 0,50,300 mmol / L） of Na Cl and KCl,O2.-production in plants from dimorphic seeds increased non- significantly with time increasing,no significant difference was observed between the two types of plants; MDA level increased significantly with the time increasing under salt stress,and Na Cl stress rose more obviously. The activity of the antioxidativ enzymes（ SOD,CAT,POX） was increased,and the CAT and POX were much higher; moreover,Na Cl was more effective than KCl,the lower concentration of salt was more effective than the higher concentration,in promotion of the dimorphic plants with high enzymatic activity. All param-eters above showed no significant difference between two types of plants.【Conclusion】The results indicated antioxidative enzymes played an important role in short- term salt tolerance in C. album; the plants from the two types of seeds displayed similar physiological and biochemical characteristics under short- term salt stress.

关 键 词：藜 异型性种子后代植株 抗氧化系统 盐胁迫 

分 类 号：S181[农业科学—农业基础科学]