大豆GmWRI1基因在糖、植物激素及盐胁迫下的表达分析  

Analysis of the Expression of Soybean Gm WRI1 Gene under Sugar,Phytohormone and Salt Stress

作　　者：李丹丹[1] 闫丽[1] 常健敏 王志坤[1] 李文滨[1] 

机构地区：[1]东北农业大学、大豆生物学教育部重点实验室、农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《作物杂志》2015年第4期41-46,共6页Crops

基　　金：优质功能型转基因大豆新品种培育(2014ZX08004-003);东北农业大学博士启动金;黑龙江省教育厅面上项目(11551045)

摘　　要：大豆是我国重要油料作物之一,提高大豆的产油量一直是大豆育种的重要方向。WRIN-KLED(WRI)作为AP2/EREBP家族的一个转录因子,在油脂合成过程中参与油脂合成和积累。2014年对大豆东农47进行葡萄糖(Glucose)、蔗糖(Sucrose)、赤霉素(gibberellin A3,GA3)、脱落酸(abscisic acid,ABA)、盐(Na Cl)等外源处理,分析不同处理Gm WRI1基因的表达。研究表明,Gm WRI1基因受葡萄糖、赤霉素、ABA信号诱导,为这3种信号途径中的正向调控因子;在蔗糖、氯化钠处理中,Gm WRI1基因表达量下调,为蔗糖、盐胁迫中的负调控因子。为后续深入研究大豆油脂合成调控提供一定的理论基础。Soybean [ Glycine max （ L. ） Merr. ] has a huge market in China. It is one of the most important oil crops in China. With its unique flavor and quality, soybean oil is widely loved by the people, especially the people in northern of China. Improving oil production of soybean has been an important research direction in soybean breed- ing. The current study showed that as one of the AP2/EREBP transcription factor family, WRINKLED （WRI） partic- ipated in oil synthesis and accumulation. Exogenous glucose, sucrose, gibberellin （ gibberellin A3, GA3 ）, abscisic acid （ABA）, salt treatment on soybean had shown that the GmWRI1 gene was regulated by glucose, GA3, ABA sig- nal. It is a positive regulation factor in these three signaling pathways. As the negative control factor of sucrose and salt stress, the expression of GmWRI1 gene decreased in the sucrose, and sodium chloride treatment. This experiment provided comprehensive and in-depth understanding for the further studies on synthetic control of soybean oil.

关 键 词：大豆 糖 盐 植物激素处理 高油 转录因子 GmWRll 

分 类 号：S565.1[农业科学—作物学]