Study on Multiplication, Rooting and Transplanting of Tissue Culture Plantlets of Rhododendron chrysanthum Pall  

牛皮杜鹃组培苗的增殖、生根培养及炼苗移栽研究（英文）

作　　者：王蕾[1] 巴春影[1] 曹后男[1] 宗成文[1] 姚航[1] 

机构地区：[1]延边大学农学院园艺园林系,吉林延边133002

出　　处：《Agricultural Science & Technology》2015年第7期1348-1351,1373,共5页农业科学与技术（英文版）

基　　金：Supported by Students'Innovation and Entrepreneurship Training Program of Yanbian University in 2015(ydbksky2015252)~~

摘　　要：[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA （3 mg/L） and ZT （1.5 mg/L） was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium （150 mg/L）. [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS （1/4） and IBA （5.0 mg/L）, and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite （2：1：1） with a survival rate of 95.66%.[目的]该研究旨在缩短长白山牛皮杜鹃增殖、生根培养周期。[方法]以长白山牛皮杜鹃组培苗为试材,研究了不同激素组合以及椰乳对牛皮杜鹃组培苗增殖分化的影响,探究了牛皮杜鹃组培苗生根培养基、驯化移栽基质。[结果]改良MS+IBA 3 mg/L+ZT 1.5mg/L是牛皮杜鹃组培苗继代增殖的最佳培养基,且在培养基中加入150 ml/L椰乳可明显促进其增值倍数和植株平均高度。牛皮杜鹃组培苗最佳生根培养基为1/4改良MS+IBA5.0 mg/L,接种后8 d即开始生根。沙培15 d进行催根处理,适宜基质为草炭土∶腐殖土∶珍珠岩=2∶1∶1,成活率为95.66%。

关 键 词：Rh. chrysanthum Pall Multiplication culture ROOTING Transplanting matrix 

分 类 号：S685.21[农业科学—观赏园艺]