桃组织培养外植体灭菌方法的筛选  

Disinfection methods selection of peach explants in fast reproduction system

作　　者：方庆[1] 熊明国[1] 决超[1] 

机构地区：[1]商丘职业技术学院,河南商丘476000

出　　处：《中国园艺文摘》2015年第7期36-37,共2页Chinese Horticulture Abstracts

基　　金：河南省科技转化项目(30400224)

摘　　要：试验采用75%乙醇、Hg Cl2(升汞)和Na Cl O(次氯酸钠)作为灭菌试剂,通过对‘桃豫矮化砧木1号’茎段灭菌效果的比较,探讨合理有效的灭菌方法。结果表明:用75%乙醇与不同浓度的Hg Cl2(升汞)混合使用的灭菌效果要明显好于75%乙醇与Na Cl O(次氯酸钠)混合使用的灭菌效果。其中,以75%乙醇灭菌30 s+0.15%Hg Cl2(升汞)灭菌9 min效果最好。The stem segments of the dwarf Yunong rootstock No.1 was disinfected by 75% alcohol, NaClO and HgCl2 to discuss the reasonable and effective disinfection methods. The results indicated that the disinfection effect of 75% alcohol and HgCl2 was better than 75% alcohol and NaClO. The best method is 75% alcohol 30 s and 0.15% HgCl2 9 minutes.

关 键 词：桃 矮化砧木 升汞 乙醇 次氯酸钠 组织培养 

分 类 号：S682.11[农业科学—观赏园艺]