临床微生物实验室细菌分离接种技术的研究进展  

Research progress on the bacterial inoculation techniques in clinical microbiology laboratories

作　　者：王敬华 葛平 陈蓉 徐蓉 刘学杰 

机构地区：[1]上海市临床检验中心,上海200126

出　　处：《检验医学》2015年第7期757-760,共4页Laboratory Medicine

基　　金：上海市自然科学基金资助项目(15ZR1436100);上海市卫生和计划生育委员会重要疾病联合攻关项目(2013ZYJB0010);上海市卫生和计划生育委员会重点研究项目(20134010)

摘　　要：近年来,自动化研究方兴未艾,出现了商品化的自动化、半自动化微生物接种仪,如Robobact system全自动微生物分离培养系统、Easy Spiral Pro全自动螺旋接种仪以及PREVI Isola自动化微生物接种仪等。然而临床实践表明,目前尚没有比较理想的自动化接种仪,特别是能够准确定量接种的自动化仪器,致使现有商品化自动接种仪在临床实验室难以推广。同时,建立细菌培养定量检验方法,进一步统一和标准化操作程序,已成为临床微生物检验质量控制的发展趋势。基于伯努利原理和流体力学,国内学者设计研发的多通道自动化微生物接种仪,有望建立基于自动化接种技术的细菌培养定量检验方法。现就临床微生物实验室细菌分离接种技术的发展现状、趋势和研究进展进行综述。In recent years,researches on the bacterial inoculation automation are booming. The commercialized automatic or semi-automatic microbial inoculation instruments are developing,such as,Robobact system,Easy Spiral Pro and PREVI Isola. However,the experience of clinical application indicates that no instrument employed is satisfactory,let alone the accurate quantitative inoculation instrument,and they are hard to be popularized in clinical microbiology laboratories. Meanwhile,to develop a new method of accurate quantitative determination and to unify and standardize operating procedure,is the development tendency of quality control. Based on Bernoulli principle and fluid mechanics,a new-type instrument,called the multichannel microbial inoculation instument,is invented by Chinese scholars. It is hopeful to develop a new method for the quantitative determination for microbiology based on automatic technique. In this review,the research and development progress of bacterial inoculation technique in clinical microbiology laboratories are summarized.

关 键 词：接种技术 临床微生物 细菌培养 自动化 定量分析 

分 类 号：R446.5[医药卫生—诊断学]