山羊ZP3基因的cDNA克隆、序列分析及组织表达研究  

cDNA Cloning,sequence analysis and tissue expression of goat ZP3 gene

作　　者：刘霜[1] 卢建远[1] 马力[1] 夏威[1] 胡亮[1] 罗斌[1] 杨珂伟 字向东[1] 

机构地区：[1]西南民族大学国家民委动物科学重点实验室,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2015年第4期412-416,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省应用基础项目(2013JY0043);西南民族大学研究生创新型科研项目(CX2014SZ69)

摘　　要：对高繁金堂黑山羊和低繁藏山羊ZP3基因c DNA进行克隆、序列分析,并采用Real-time PCR技术对其在发情前期母羊卵巢组织的表达进行研究.结果表明:山羊ZP3基因编码区全长为1269bp,共编码422个氨基酸,两品种间有2处碱基差异,但未导致氨基酸的差异.山羊的ZP3基因在卵巢表达量高,与其他组织差异极显著(P<0.01),但两个品种间差异不显著(P>0.05).说明ZP3基因主要在卵巢中表达,但可能不是影响山羊多羔性状的主基因.This study was carried out for ZP3 gene cloning and sequence analysis on non-prolific Tibetan goats and prolific Jin- tang black goats, and its mRNA expression levels in ovary at pro-estrus were determined by real-time PCR. The result showed that the coding region sequence（CDS） of goat ZP3 gene was 1269bp long,encoding 422 amino acids. There were 2 basic differ- ences between the two breeds without amino acid change. The mRNA expression level was higher in ovary than in other tissues （P 〈0.01 ）, but there was no differential expression between the two breeds（ P 〉 0.05 ）. The result shows that ZP3 gene is highly expressed in ovary, but it may not be the major gene to determine the kidding rate in goat.

关 键 词：山羊 ZP3 克隆 序列分析 qPCR 

分 类 号：S814[农业科学—畜牧学] S827[农业科学—畜牧兽医]