氮磷营养盐因子对缘管浒苔生长、叶绿素荧光特性和氮磷富集的影响  

Effects of Nitrogen and Phosphorus Nutrients on Growth,Photosynthesis and Enrichment of Nitrogen and Phosphorus of Ulva linza

作　　者：李文慧[1,2] 朱明[1,2] 刘冉[1] 申振兴 赵海燕[1] 王长海[1] 郑青松[1,2] 郑春芳[4] 

机构地区：[1]南京农业大学资源与环境科学学院/江苏省海洋生物学重点实验室,江苏南京210095 [2]淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港222005 [3]南京农业大学生命科学学院,江苏南京210095 [4]浙江省海洋水产养殖研究所浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005

出　　处：《生态与农村环境学报》2015年第4期553-558,共6页Journal of Ecology and Rural Environment

基　　金：淮海工学院江苏省海洋生物技术重点建设实验室研究基金(2012HS012);江苏高校优势学科建设工程资助项目(CXTD21);南京农业大学院级SRT项目(201219)

摘　　要：为探讨大型海藻缘管浒苔(Ulva linza)对氮、磷加富的生理响应及其机制,分析了氮、磷浓度变化对藻体相对生长速率(Rr,g),氮、磷富集,叶绿素(Chl)含量,类胡萝卜素(Car)含量,色素比值(Chl a/Chl b、Chl/Car)以及叶绿素荧光参数的影响。结果表明,在30μmol·L-1P浓度不变条件下,随着N浓度的增加,藻体P含量持续降低,而其Rr,g、N含量、Chl含量、Car含量、色素比值(Chl a/Chl b、Chl/Car)和叶绿素荧光参数均逐渐上升,N3处理(500μmol·L-1N)缘管浒苔Rr,g和叶绿素荧光参数均达到最大值,N4处理(1 000μmol·L-1)缘管浒苔Chl含量、Car含量和Chl a/Chl b比值均达到最大值。在500μmol·L-1N浓度不变条件下,依次增加P浓度,缘管浒苔Rr,g没有显著差异,N含量没有显著变化,而P含量则呈明显上升趋势,其他指标变化幅度小。综上所述,与P相比,N的变化对缘管浒苔生长、光合色素和光合作用的影响更明显,在N浓度为500μmol·L-1、P浓度为30μmol·L-1、N/P比值为16.67条件下,藻体生长最佳。当水体富营养化加剧时,缘管浒苔富集氮、磷的能力持续上升。To investigate physiological responses of Ulva linza to addition of N and P and its mechanisms,an experiment was conducted culturing Ulva linza in solutions different in N and P concentration for 10 days and then relative growth rate（ Rr,g）,contents of N,P,chlorophyll（ Chl） and carotenoid（ Car）,Chl a/Chl b,Chl/Car and chlorophyll fluorescence parameters（ CFP） were determined. Results show that in the solutions kept the same in P concentration（ 30 μmol·L- 1P）,with rising N concentration,all the indices of the algae gradually increased except for P content which declined steadily,and both Rr,gand CFP peaked and when N content reached 500 μmol·L- 1,and Chl,Car and Chl a/Chl b did when N content reached 1 000 μmol·L^- 1. However,in the solutions kept the same in N concentration（ 500 μmol·L^- 1N）,rising P concentration did not affect Rr,gor N content in the algae much,however,it did increase P content significantly in the algae,while the other indices varied just slightly. To sum up,compared with P,N is more important to growth,formation of photosynthetic pigment and photosynthesis of Ulva linza. In the solution with 500 μmol·L-1N and 30 μmol·L^- 1P（ N/P 16. 67）,U. linza grows the most healthily and when the water body is getting eutrophied,U. linza N and P enrichment capacity rises steadily,too.

关 键 词：缘管浒苔 氮 磷 生长 叶绿素荧光特性 

分 类 号：X52[环境科学与工程—环境工程] Q175[生物学—水生生物学]