Stability of rat models of fluid percussion-induced traumatic brain injury: comparison of three different impact forces  

作　　者：Yun-peng Lin Rong-cai Jiang Jian-ning Zhang 

机构地区：[1]Department of Neurosurgery, Tianjin Medical University General Hospital [2]Tianjin Neurological Institute [3]Key Laboratory of Post-trauma Neurorepair and Regeneration in Central Nervous System, Ministry of Education [4]Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System [5]Tianjin Medical University General Hospital

出　　处：《Neural Regeneration Research》2015年第7期1088-1094,共7页中国神经再生研究（英文版）

基　　金：supported by a grant from the International S cience and Technology Cooperation Projects of China,No.2011DFG33430

摘　　要：Fluid percussion-induced traumatic brain injury models have been widely used in experimental research for years. In an experiment, the stability of impaction is inevitably affected by factors such as the appearance of liquid spikes. Management of impact pressure is a crucial factor that determines the stability of these models, and direction of impact control is another basic element. To improve experimental stability, we calculated a pressure curve by generating repeated impacts using a fluid percussion device at different pendulum angles. A stereotactic frame was used to control the direction of impact. We produced stable and reproducible models, including mild, moderate, and severe traumatic brain injury, using the MODEL01-B device at pendulum angles of 6°, 11° and 13°, with corresponding impact force values of 1.0 ± 0.11 atm（101.32 ± 11.16 k Pa）, 2.6 ± 0.16 atm（263.44 ± 16.21 k Pa）, and 3.6 ± 0.16 atm（364.77 ± 16.21 k Pa）, respectively. Behavioral tests, hematoxylin-eosin staining, and magnetic resonance imaging revealed that models for different degrees of injury were consistent with the clinical properties of mild, moderate, and severe craniocerebral injuries. Using this method, we established fluid percussion models for different degrees of injury and stabilized pathological features based on precise power and direction control.Fluid percussion-induced traumatic brain injury models have been widely used in experimental research for years. In an experiment, the stability of impaction is inevitably affected by factors such as the appearance of liquid spikes. Management of impact pressure is a crucial factor that determines the stability of these models, and direction of impact control is another basic element. To improve experimental stability, we calculated a pressure curve by generating repeated impacts using a fluid percussion device at different pendulum angles. A stereotactic frame was used to control the direction of impact. We produced stable and reproducible models, including mild, moderate, and severe traumatic brain injury, using the MODEL01-B device at pendulum angles of 6°, 11° and 13°, with corresponding impact force values of 1.0 ± 0.11 atm（101.32 ± 11.16 k Pa）, 2.6 ± 0.16 atm（263.44 ± 16.21 k Pa）, and 3.6 ± 0.16 atm（364.77 ± 16.21 k Pa）, respectively. Behavioral tests, hematoxylin-eosin staining, and magnetic resonance imaging revealed that models for different degrees of injury were consistent with the clinical properties of mild, moderate, and severe craniocerebral injuries. Using this method, we established fluid percussion models for different degrees of injury and stabilized pathological features based on precise power and direction control.

关 键 词：nerve regeneration traumatic brain injury fluid percussion impact force pressure curve head fixed impact peak animal models neural regeneration 

分 类 号：R651.15[医药卫生—外科学] R-332[医药卫生—临床医学]