金属特性碳纳米管的筛选分离及对人类乳腺癌细胞的光热效应  

Photothermal effects of metallic carbon nanotubes on human breast cancer cells

作　　者：侯进[1] 弥曼[1] 魏明[1] 易文辉[2] 

机构地区：[1]西安医学院基础医学部药理学与毒理学教研室,陕西西安710021 [2]西安交通大学电信学院陕西省信息光子技术重点实验室,陕西西安710049

出　　处：《药学学报》2015年第8期980-985,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(61275179);陕西省教育厅专项科研项目(2010JK805);西安医学院博士科研启动基金资助项目(2011DOC04);西安交通大学交叉学科项目(XJJ2015112)

摘　　要：分离并研究金属碳纳米管(m-SWNTs)的光热效应及其对乳腺癌细胞的抑制作用。采用Pm PV(poly[(m-phenylenevinylene)-alt-(p-phenylenevinylene)])从单壁碳纳米管(SWNTs)中分离得到具有金属特性碳纳米管,测定其光热效应,与MCF-7乳腺癌细胞共同培养,在808 nm波长下,用红外光(NIR)以2 W·cm-2的强度照射细胞3 min,MTT比色法测定m-SWNTs对MCF-7细胞的杀灭作用;流式细胞术测定m-SWNTs对MCF-7细胞凋亡的影响。结果表明,用Pm PV可有效分离m-SWNTs,其光热效应明显增强;可以抑制MCF-7细胞的存活率,促进MCF-7细胞凋亡,其效果强于未经分离的SWNTs。通过Pm PV分离的m-SWNTs具有更高的光热转化效率,并且对MCF-7细胞具有更强的杀灭效果。SWNTs are a mixture of 1/3 metallic SWNTs （m-SWNTs） and 2/3 semiconducting SWNTs （s-SWNTs）. It is desirable to separate the metallic SWNTs from the semi-condncting ones. In this study m-SWNTs was separated by using a poly[（m-phenylenevinylene）-alt-（p-phenylenevinylene）] （PmPV） derivative and used as photo-thermal media instead of SWNTs. The separation effects of m-SWNTs were evaluated by Raman spectra, molecular modeling and TEM images. The effects of m-SWNTs on MCF-7 cell proliferation and apoptosis were evaluated with MTT assay and flow cytometry, respectively, m-SWNTs were separated with high purity. A strong inhibition of MCF-7 cell growth was observed with the m-SWNTs under near-infrared （NIR） light irradiation, Our results will be helpful for the potential applications of m-SWNTs in clinical photo- thermal cancer therapy.

关 键 词：碳纳米管 MCF-7细胞 光热效应 

分 类 号：R963[医药卫生—微生物与生化药学]