采用HPLC方法检测卟啉类光敏剂的研究  

Analysis of porphyrin photosensitizers using HPLC method

作　　者：贾敏鸽[1] 吴海燕 孙丽丽[1] 姚春所[1] 张绍良 李亚伟 方起程[1] 

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,北京100050 [2]青龙高科技股份有限公司,江西宜春336000

出　　处：《药学学报》2015年第8期1021-1025,共5页Acta Pharmaceutica Sinica

基　　金：国家十二五“重大新药创制”科技重大专项(2012ZX09103101-052)

摘　　要：光动力学疗法(photodynamic therapy,PDT)具有良好的靶向性、微创性和安全性,已成为肿瘤防治中的重要领域,而光敏剂(photosensitizer)则是PDT的核心和关键。本文报道了一种用于卟啉类光敏剂的新HPLC分析方法,采用Shiseido Capcell PAK C18(150 mm×4.6 mm,5μm)色谱柱,柱温30℃,甲醇-1%冰乙酸溶液为流动相,流速1.0 m L·min-1,梯度洗脱,检测波长380 nm。方法学研究表明本方法具有良好的专属性、精密度、准确度和重复性,适用于卟啉类光敏剂的纯度检查和含量测定,已在新型光敏剂华卟啉钠的开发中发挥了重要导向作用。Photodynamic therapy （PDT）, because of its good targeting, minimal invasion, and safety, is becoming a very active area in cancer prevention and treatment, in which the photosensitizers have proved to be the core element for PDT. We developed a new HPLC method for analyzing porphyrin photosensitizers using Shiseido Capcell PAK C18 （150 mm ×4.6 mm, 5 μm） as the column at 30 ℃, methanol-1% aqueous solution of acetic acid as the mobile phase in a flow rate of 1.0 mL·min^-1 in a gradient elution mode, and the detection wavelength at 380 nm. This method, showing good specificity, precision, accuracy and robusty via methodology validations, can be applied to the purity test and assay of porphyrin photosensitizers, and has played a key guide role in the R＆D of the new porphyrin photosensitizer — sinoporphyrin sodium.

关 键 词：光动力学疗法 光敏剂 卟啉衍生物 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]