宁心痛颗粒对人类单核细胞株源性巨噬细胞泡沫化的影响  

Effect of Ningxintong Granule(宁心痛颗粒) on Human Monocyte Derived Macrophage Foam

作　　者：都基莎[1] 顾宁[2] 

机构地区：[1]中国人民解放军第四五五医院、上海市长宁区淮海西路338号,200052 [2]南京中医药大学第三附属医院

出　　处：《中医杂志》2015年第15期1322-1325,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81173399)

摘　　要：目的探讨宁心痛颗粒干预动脉粥样硬化易损斑块的可能作用机制。方法 SD大鼠分别灌胃宁心痛颗粒3、15、30、60 g/(kg·d)浓煎剂4天,制备宁心痛颗粒等剂量、5倍、10倍、20倍含药血清。实验分为巨噬细胞组、模型组及宁心痛颗粒含药血清等剂量5倍、10倍、20倍剂量组。除巨噬细胞组外其余各组采用氧化低密度脂蛋白体外诱导正常生长的人类单核细胞株(THP-1)细胞建立巨噬细胞泡沫化模型。宁心痛颗粒含药血清各剂量组分别加入相应剂量的含药血清,使含药血清终浓度为3%,每组实验重复4次。运用Western blot法检测各组细胞脂蛋白受体清道夫受体A(SRA)、CD36蛋白表达水平。结果模型组细胞SRA、CD36蛋白表达含量较巨噬细胞组明显增高(P<0.05);宁心痛颗粒含药血清各剂量组细胞SRA、CD36蛋白水平均较模型组明显降低(P<0.01);宁心痛颗粒含药血清10倍剂量组细胞SRA、CD36的蛋白表达水平低于宁心痛颗粒其他剂量组(P<0.01)。结论宁心痛颗粒能够下调巨噬细胞SRA、CD36蛋白表达,从而抑制泡沫细胞的形成,可能是其干预动脉粥样硬化易损斑块的分子机制之一。Objective To explore possible mechanism of Ningxintong Granule acting on vulnerable atherosclerotic plaques. Methods Sprague-Dawley （SD） rats were given concentrated decoction of Ningxintong Granule 3, 15, 30, 60g/（kg · d） by gavage for 4 days. Prepare serum medicated Ningxintong Granule isodose, 5 times, 10 times and 20 times. There were macrophages group, model group, serum medicated Ningxintong Granule isodose, 5 times, 10 times and 20 times groups. Besides macrophages group, foam cells models in other groups were established by normally grown human monocytic cell line （ THP-1 ） cells in vitro induction by oxidized low density lipoprotein. Serum medicated Ningxintong Granule groups were added medicated serum of corresponding dose and final concentrations were 3%. Test 4 times in each group. Detect the expression of scavenging receptor A （SRA） and CD36 in each group. Results The expression of SRA and CD36 in model group was more than that in macrophages group （P 〈 0. 05 ）. The expression of SRA and CD36 in serum medicated Ningxintong Granule groups was less than that in model group （ P 〈 0. 01 ）. The expression of SRA and CD36 in serum medicated Ningxintong Granule l0 times group was less than that in other dose groups （P 〈 0. 01 ）. Conclusion Ningxintong Granule could decrease the expression of SRA and CD36 and inhibit foam cells formation, which may be one of the molecular mechanisms of Ningxintong Granule acting on vulnerable atherosclerotic plaques.

关 键 词：宁心痛颗粒 巨噬细胞 泡沫细胞 动脉粥样硬化 易损斑块 清道夫受体 

分 类 号：R285.5[医药卫生—中药学]