黄精多糖PSP-1-A的分离纯化及结构解析  

Separation,Purification,and Structural Analysis of PSP-1-A of Polygonatum Sibiricmu Polysaccharides

作　　者：李舒婕[1] 林海桢 施胜英 林敬明[1] 

机构地区：[1]南方医科大学珠江医院,广东广州510282

出　　处：《河南中医》2015年第6期1441-1445,共5页Henan Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2012A030100008)

摘　　要：目的:探讨黄精多糖的提取及分离纯化条件,对得到的单一多糖进行分子量测定、单糖组成分析,并对其一级结构进行初步分析。方法:制黄精经石油醚脱脂后,采用水提醇沉,DEAE-cellulose及葡聚糖凝胶(Sephadex G-200)色谱柱分离纯化;高效液相色谱法进行纯度鉴定及分子量测定;气质联用测单糖组成;红外光谱和核磁共振对其结构进行分析。结果:获得一种多糖(PSP-1-A),数均分子量为:1410,重均分子量(Mw)为:8640,分布宽度(Mw/Mn)为:6.12。单糖主要由半乳糖组成,另外含有少量葡萄糖(Glc)、鼠李糖(Rha)、木糖(Xyl)、阿拉伯糖(Ara)及甘露糖(Man),其摩尔比为93.8∶1.4∶1.3∶1.5∶1.1∶0.9。红外及核磁结构分析证明PSP-1-A是由β-1,4-半乳糖(gal)和β-T-半乳糖(gal)构成的线性多糖。结论:PSP-1-A是一种全新单糖组成的相对分子量均一的黄精多糖。Objective： To discuss the extraction,separation and purification conditions of polygonatum sibiricmu polysaccharides,determine the molecular weight of the single polysaccharide,analyze the composition of the monosaccharide,and further preliminarily analyze its primary structure. Methods： Degrease the propared rhizoma polygonati by petroleum ether,apply water solution and alcohol sedimentation,DEAE-cellulose and Sephadex G-200 chromatographic column separation and purification,identify the purity with high performance liquid chromatography（ HPLC）,determine the molecular weight,detect the composition of monosaccharide with GC-MS,analyze the structure with infrared spectroscopy and nuclear magnetic resonance. Results： One polysaccharide was got with the molecular weight of 1410,the Mw was 8640,and the Mw / Mn was 6. 12. The monosaccharide was mainly composed by galactose and small amount of glc,rha,xyl,Ara and Man,with the molar ratio of 93. 8∶1. 4∶1. 3∶1. 5 ∶1. 1 ∶0. 9.The analysis of infrared spectroscopy and nuclear magnetic resonance indicates that PSP-1-A was the linear polysaccharide composed by β-1,4-gal and β-T-gal. Conclusion： PSP-1-A is a kind of polygonatum sibiricmu polysaccharide composed by new monosaccharide and of uniform relative molecular weight.

关 键 词：黄精 多糖 单糖组成 结构解析 

分 类 号：R284.2[医药卫生—中药学]