成年绵羊不同组织中GPR 30 mRNA表达特性研究  

GPR 30 m RNA Expression in Different Tissues of Adult Sheep

作　　者：路佩瑶[1] 曹宁贤 王福传[1] 宋献艺[1] 闫益波[3] 高莉[4] 赵英虎[4] 张凯[1] 张玉换[3] 

机构地区：[1]山西省农业科学院饲料兽药研究所,太原030031 [2]山西省畜禽繁育工作站 [3]山西省农业科学院畜牧兽医研究所,太原030032 [4]中北大学化工与环境学院

出　　处：《中国草食动物科学》2015年第4期13-16,共4页China Herbivore Science

基　　金：山西省农业科技攻关项目(20130311025-1);山西省科技攻关项目(20140311008-7);山西省农业科技攻关项目(20130311025-4);山西省农业科学院博士基金项目(YBSJJ1403);山西省青年科技研究基金(2012021027-3)

摘　　要：文章旨在研究成年绵羊不同组织中GPR 30 m RNA的表达特性。采用q RT-PCR法对成年杂交绵羊不同组织中GPR 30 m RNA的表达进行相对定量分析。结果表明:GPR 30 m RNA在心脏等其他组织中有少量的表达,在卵巢、附睾尾及下丘脑中显著表达(P<0.05)。说明GPR 30 m RNA在成年绵羊的不同组织中均有不同程度的表达,并在繁殖器官的生长、发育过程中起着重要的作用。The objective of this study was to evaluate GPR 30 mRNA expression in different tissues of adult sheep. GPR 30 mRNA expressions in different tissues of adult sheep were detected by real-time fluorescent quantitative PCR. The results showed that GPR 30 mRNA was not significantly expressed in the heart and other organizations. However, GPR 30 mRNA was significantly expressed in the ovary, epididymidis body and hypothalamus （P 〈 0.05 ）. GPR 30 mRNA appeared to be ubiquitously expressed almost in different tissues of adult sheep. It plays an important role in the sheep reproductive organ growth and development.

关 键 词：绵羊 GPR30 卵巢 睾丸 下丘脑 

分 类 号：S826.2[农业科学—畜牧学]