黄芩药材不同取样部位指纹图谱研究  

STUDY ON THE FINGERPRINT OF DIFFERENT SAMPLING PARTS OF SCUTELLARIA BAICALENSIS

作　　者：赵胜男[1] 李艳荣[1] 申川川 李守拙[1] 

机构地区：[1]河北省中药研究与开发重点实验室/承德医学院中药研究所,河北承德067000

出　　处：《承德医学院学报》2015年第4期284-286,共3页Journal of Chengde Medical University

摘　　要：目的:考察黄芩不同取样部位10种黄酮类成分含量的变化。方法:使用TC-C18色谱柱(4.6mm×250mm,5μm,Agilent),流动相:乙腈与0.1%冰醋酸溶液进行梯度洗脱,检测波长276nm,流速为1.0ml/min,柱温25℃,以面积归一化法对数据进行分析。结果:秋季三年生黄芩不同取样部位10种黄酮类成分的含量比例各不相同,苷类成分含量从上到下越来越低、苷元类成分含量从上到下越来越高。结论:黄芩不同取样部位黄酮类成分的含量不同,应注意保证取样的科学性。Objective： To investigate the changes of 10 kinds flavonoids contents in different sampling parts of Scutellaria baicalensis. Methods： TC-C18 chromatographic column was used in this study： gradient elution with acetonitrile and 0.1% glacial acetic acid as mobile phase, detection wavelength was 276 nm, flow rate was 1.0ml/min, column temperature was 25℃. The data was analyzed by area normalization method. Results： The content ratio of 10 kinds flavonoids was different in different sampling parts of third-year Scutellaria baicalensis in fall： from top to bottom, glycoside gradually decreased, while aglycone gradually increased. Conclusions： The flavonoids contents are different in different sampling parts of Scutellaria baicalensis, so researchers should ensure the scientific nature of sampling.

关 键 词：黄芩 含量比例 面积归一化法 取样部位 

分 类 号：R917[医药卫生—药物分析学]