发育早期和成年大鼠背侧纹状体内CRF轴突终末的分布和变化  

The distribution patterns of CRF axon terminals in the developing and adult rat dorsal striatum

作　　者：许本柯[1] 孙安邦[1] 何云[1] 周晓娟[1] 刘兵[1] 陈运才[1,2] 

机构地区：[1]长江大学医学院解剖学教研室,荆州434000 [2]University of California,Irvine,CA 92697

出　　处：《神经解剖学杂志》2015年第4期497-504,共8页Chinese Journal of Neuroanatomy

基　　金：湖北省“楚天学者计划”基金(2012-12)

摘　　要：目的：探讨背侧纹状体（dorsalstriatum）内促肾上腺皮质激素释放因子（CRF）阳性轴突终末和CRF表达的分布特征及年龄变化。方法：采用免疫组织化学、原位杂交、免疫印迹和体视学分析方法，观察分析出生后第1～21日龄（P1-21）及成年（P90）大鼠背侧纹状体内的CRF轴突终末及CRF蛋白含量。结果：背内、背外侧纹状体内含大量CRF轴突终末，背外侧纹状体内的CRF轴突终末更为丰富。这些终末集中位于纹状体的尾部区域，与纹状体主要神经元形成典型的环胞体支配。和成年比较，发育早期（P1—14）背侧纹状体内CRF轴突终末的密度较高（p〈0．05），CRF蛋白表达量以P7组相对最高（P〈0．05）。结论：发育早期背侧纹状体内富含CRF轴突终末，提示CRF是影响纹状体神经元生长发育的因子之一。Objective： To obseve distribution of corticotropin-releasing factor （CRF） in the dorsal striatum. Methods： Immunohistochemistry, in situ hybridization, Western Blotting and stereological analysis were employed to investigate CRF axonal terminals and CRF expression in the dorsal stratum in immature （postnatal day 1 to 21, P1-21 ） and young adult （PgO） rats. Results： Numerous CRF axonal terminals were detected in the dorsal striatum, including the dorsolateral and dorsomedial parts. Particularly, the CRF axonal terminals were distributed in the posterior regions of the dorsal striatum, and they surrounded the cell bodies of spiny striatum neurons to form a typical perlsomatie innervation. CRF axonal termi- nals are abundant during early development （P1-P14） , and CRF expression was on the highest level in P7 as compared with the adult and others （ P 〈 0.05 ）. Conclusion： Robust expression and axonal terminals of CRF are observed in the developing dorsal striatum, suggesting that CRF might be one of factors involving in the differentiation.

关 键 词：纹状体 促肾上腺皮质激素释放因子 发育 大鼠 

分 类 号：R338[医药卫生—人体生理学]