丁布胁迫对亚洲玉米螟氧化还原系统的影响  

Effects of DIMBOA stress on oxidation reduction system of the Asian corn borer,Ostrinia furnacalis( Guenée)( Lepidoptera: Crambidae)

作　　者：毛培[1] 王甜甜[1] 宋鹏飞[1] 郭线茹[1] 原国辉[1] 罗梅浩[1] 

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《植物保护学报》2015年第4期584-590,共7页Journal of Plant Protection

基　　金：国家公益性行业(农业)科研专项(HY201203100)

摘　　要：为探明丁布胁迫对亚洲玉米螟氧化还原系统的影响,采用酶活力测定方法和实时荧光定量PCR技术,研究了取食丁布含量差异显著的3个玉米品种登海662、浚单20和隆玉602叶片及模拟其叶片丁布含量的人工饲料后,玉米螟3龄幼虫氧化还原系统主要酶活性和谷氧还蛋白(Grx)mRNA转录表达的变化。结果显示:取食3个玉米品种叶片24 h后,亚洲玉米螟幼虫过氧化物酶和超氧化物歧化酶活性均显著高于对照,且在3个品种处理间差异显著,由大到小依次为登海662(118.30 U/mg和182.19 U/mg)、浚单20(107.13 U/mg和90.02 U/mg)和隆玉602(77.54 U/mg和93.59 U/mg)。取食浚单20可显著促进幼虫脂肪体Grx的表达,取食登海662可显著促进其中肠Grx的表达;取食模拟登海662叶片丁布含量的人工饲料12 h后,幼虫脂肪体中Grx表达量高于对应玉米叶片饲喂处理,而中肠部位则低于对应玉米叶片饲喂处理;取食模拟浚单20的结果与模拟登海662相反。表明亚洲玉米螟氧化还原系统在防御丁布胁迫过程中发挥着重要作用。To investigate the effect of DIMBOA stress on oxidation reduction system of the Asian corn borer,Ostrinia furnacalis（ Guenée）,the changes of main enzyme activities in oxidation reduction system and Grx transcript of third instar O. furnacalis larvae were analyzed by enzyme activity analysis and realtime PCR methods. O. furnacalis larvae were fed with the leaves of three maize varieties（ Denghai 662,Xundan 20 and Longyu 602） with different DIMBOA contents and corresponding artificial diets containing the same DIMBOA contents as in the three maize varieties. The results revealed that superoxide dismutase（ SOD） and peroxidase（ POD） activities were significantly different among three treatments 24 h after feeding. The highest activity was observed in the larvae fed with Denghai 662（ POD： 118. 30 U / mg and SOD： 182. 19 U / mg）,followed by larvae fed with Xundan 20（ POD： 107. 13 U / mg and SOD： 90. 02 U / mg） and Longyu 602（ POD： 77. 54 U / mg and SOD： 93. 59 U / mg）. Grx transcript in fat bodies of larvae fed with Xundan 20 and in midgut of larvae fed with Longyu 602 increased significantly. Grx transcript in fat bodies of larvae fed with artificial diets containing the same DIMBOA as in Denghai 662 leaves was significantly higher 12 h after feeding; however,it began to decrease in midgut,and reversed results were found in the treatments with artificial diets containing the same DIMBOA as in Xundan 20 leaves. Altogether,these results indicated that the oxidation reduction system of O. furnacalis plays animportant role in defending DIMBOA stress.

关 键 词：丁布 亚洲玉米螟 超氧化物歧化酶 过氧化物酶 谷氧还蛋白基因 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]