辽宁省稻田野慈姑对苄嘧磺隆的抗药性  

The resistance of Sagittaria trifolia populations in rice fields from Liaoning Province to bensulfuron-methyl

作　　者：李平生[1] 魏松红[1] 纪明山[1] 东琴 王海宁[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866

出　　处：《植物保护学报》2015年第4期663-668,共6页Journal of Plant Protection

基　　金：国家公益性行业(农业)科研专项(201303031);辽宁水稻产业体系专项资金(辽农科〈2013〉271号)

摘　　要：为明确辽宁省不同稻田区野慈姑对苄嘧磺隆的抗性水平,整株测定了辽宁省大石桥(种群R1)、海城(种群R2)、苏家屯(种群R3)和开原(种群R4)共4个水稻产区野慈姑对苄嘧磺隆的抗性水平,并离体测定了各种群叶片体内乙酰乳酸合成酶(ALS)对苄嘧磺隆的敏感性。结果显示,种群R1和R2的抗药性相对较高,抗性指数分别为76.99和49.94,种群R3和R4抗性相对较低,抗性指数分别为12.48和16.91;离体测定结果表明较高水平的ALS活性可能与是否产生抗药性无关,种群R1、R2、R3、R4的抗性指数分别为81.86、67.48、10.56、24.86;抗药性程度依次为R1>R2>R4>R3。表明4个水稻产区野慈姑对苄嘧磺隆均产生了抗药性,而其体内ALS活性降低可能是产生抗药性的原因之一。In order to evaluate the resistance of Sagittaria trifolia in rice fields from Liaoning Province to bensulfuron-methyl,four suspected resistant S. trifolia populations from Dashiqiao（ R1）,Haicheng（ R2）,Sujiatun（ R3）,and Kaiyuan（ R4） rice areas were collected and their resistances were determined. Whole-plant bioassays and in vitro ALS assay were used to detect the resistance of S. trifolia to bensulfuron-methyl. The results showed that the resistance levels of the populations R1 and R2 were higher,with a GR50 R / S ratio of 76. 99 and 49. 94,respectively,while R3 and R4 expressed relatively low resistance level with a GR50 R / S ratio of 12. 48 and 16. 91,respectively. In vitro ALS assays revealed that there was no obvious relationship between resistance and higher ALS activity. The I50 R / S ratio of R1,R2,R3 and R4 were 81. 86,67. 48,10. 56,and 24. 86,respectively. Different populations had different resistance to bensulfuron-methyl in the following order： R1 R2 R4 R3. The results indicated that four S. trifolia populations in Liaoning had resistance to bensulfuron-methyl,and decreased ALS sensitivity was a possible cause for the resistance to S. trifolia.

关 键 词：野慈姑 苄嘧磺隆 乙酰乳酸合成酶 抗药性 

分 类 号：S481.4[农业科学—农药学]