胡桃醌对宫颈癌SiHa细胞增殖及细胞周期的影响  

Effects of Juglone on proliferation and cell cycle of human cervical cancer SiHa cells

作　　者：张巍[1] 吴亚猛 陈思瑶[2] 张琳娜[2] 韩景春[2] 那文婷[2] 金瑛[3] 

机构地区：[1]吉林医药学院生物化学教研室,吉林吉林132013 [2]吉林医药学院临床医学院,吉林吉林132013 [3]吉林医药学院药学院药物化学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2015年第4期697-700,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(21102055);吉林省科技厅科技发展计划项目资助课题(20130101157JC);吉林省教育厅"十二五"教育技术研究项目资助课题(2012-314;2014-371)

摘　　要：目的:探讨胡桃醌对人宫颈癌SiHa细胞增殖及细胞周期的影响,阐明胡桃醌在宫颈癌治疗中的作用。方法:选取处于对数生长期的SiHa细胞,随机分为对照组和10、20、40、60、80和100μmol·L-1胡桃醌处理组。倒置显微镜下观察细胞的形态学变化,噻唑蓝(MTT)法检测细胞增殖活性和细胞生长抑制率,流式细胞术检测SiHa细胞的细胞周期。结果:与对照组比较,胡桃醌处理组SiHa细胞体积缩小,连接消失,与周围细胞脱离。与对照组比较,胡桃醌处理组细胞增殖活性降低(P<0.05),细胞生长抑制率升高(P<0.05),且呈剂量依赖性,半数抑制浓度(IC50)为20.7μmol·L-1;流式细胞术检测,与对照组比较,10和100μmol·L-1胡桃醌处理组SiHa细胞的亚二倍体峰(SubG1)比率增加,G0/G1和S期细胞比率降低,G2/M期细胞比率先升高后降低。结论:胡桃醌对SiHa细胞增殖具有抑制作用,并可使细胞周期SubG1比率增加。Objective To explore the effects of Juglone on the proliferation and cell cycle of human cervical cancer SiHa cells,and to clarify the therapeutic effect of Juglone in cervical cancer.Methods The SiHa cells at logarithmic phase were divided into control group and different doses（10,20,40,60,80,100μmol·L^-1）of Juglone groups.The morphological changes of the SiHa cells were observed under inverted phase contrast microscope.The proliferation activities and the inhibitory rats of growth of SiHa cells were measured by MTT assay.The cell cycle of SiHa cells was detected by flow cytometry（FMC）.Results Compared with control group,the volume of SiHa cells reduced,the connection with the surrounding cells disappeared,and they detached from the surrounding cells in Juglone groups.Compared with control group,the proliferation activities of the SiHa cells in Juglone groups were decreased（P〈0.05）and the inhibitory rates of growth of SiHa cells were increased in a dose-dependent manner in Juglne groups;the IC50 value of Juglone was 20.7μmol·L^-1.The results of FMC showed that the ratios of SbuG1 state in Juglone groups were increased,and the ratios of SiHa cells at G0/G1 and S phases were decreased,and the ratios at G2/M phase were increased previously then were decreased in 10and100μmol·L^-1 Juglone groups compared with control group.Conclusion Juglone could inhibit the proliferation of SiHa cells and cause the increase of the SubG1 ratio of cell cycle.

关 键 词：胡桃醌 宫颈肿瘤 SIHA细胞 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R737.33[医药卫生—中医学]