银杏叶提取物各组分对高糖培养下系膜细胞增殖及细胞外基质积聚的抑制作用  

Inhibitory effects of different Ginkgo biloba leaf extract ingredients on proliferation and ECM accumulation of high glucose-treated mesangial cells

作　　者：苏青[1,2] 孙成博 孙波[2] 姚云鹏[2] 郭梦环 于晓艳[2] 

机构地区：[1]吉林大学第四医院病理科,吉林长春130011 [2]吉林大学药学院实验药理与毒理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第4期779-784,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20080141)

摘　　要：目的:探讨银杏叶提取物(GBE)不同组分对高糖培养下系膜细胞增殖及细胞外基质(ECM)积聚的作用,阐明其作用机制。方法:高糖培养的系膜细胞分别给予GBE、总黄酮、总内酯、总黄酮水解物、槲皮素、山奈酚、异鼠李素、银杏内脂A、银杏内脂B和银杏内脂C,药物浓度分别为0(高糖对照组)、3.125、6.250、12.500、25.000和50.000mg·L-1,同时设低糖对照组,MTT法检测各组系膜细胞增殖情况。高糖培养的系膜细胞分别给予GBE、总黄酮、总内酯、银杏内脂A、银杏内脂B和银杏内脂C,药物浓度分别为0(高糖对照组)、0.05、0.50、5.00和50.00mg·L-1,同时设低糖对照组,ELISA法检测条件培养基中Ⅳ型胶原(ColⅣ)和纤维连接蛋白(FN)水平。结果:MTT检测,与高糖对照组比较,50.000mg·L-1 GBE组细胞增殖活性明显降低(P<0.01),25.000和50.000 mg·L-1总黄酮组细胞增殖活性明显降低(P<0.05或P<0.01),总黄酮水解物、槲皮素、山奈酚和异鼠李素各浓度组细胞增殖活性均明显降低(P<0.01),50.000mg·L-1内脂C组细胞增殖活性明显降低(P<0.05);其他各组细胞增殖活性与高糖对照组比较差异均无统计学意义(P>0.05)。ELISA检测,与高糖对照组比较,5.00和50.00mg·L-1 GBE组ColⅣ和FN水平均明显降低(P<0.01),0.50mg·L-1 GBE组FN水平明显降低(P<0.05),0.50、5.00和50.00mg·L-1总黄酮组ColⅣ水平降低(P<0.05或P<0.01),50.00mg·L-1总内脂组ColⅣ和FN水平降低(P<0.05或P<0.01),50.00mg·L-1内脂C组FN水平降低(P<0.05)。结论:GBE在高浓度时(50.000mg·L-1)抑制高糖诱导的肾小球系膜细胞增殖而低浓度(自0.500mg·L-1起)时抑制系膜细胞ECM积聚;GBE中总黄酮在抑制细胞增殖和ECM积聚方面起主要作用,总内脂无明显效果;苷元抑制系膜细胞增殖的作用远远强于糖苷。Objective To investigate the inhibitory effects of different Ginkgo biloba leaf extract（GBE）ingredients on the proliferation and extracellular matrix（ECM）accumulation of high glucose-treated mesangial cells,and to clarify their mechanisms.Methods The high glucose-treated mesangial cells（HBZY-1）were given GBE,total flavonoids,total ginkgolide,total flavonoids hydrolyzate,quercetin,kaempferol,isorhamnetin,ginkgolide A,B,and ginkgolide C;the concentrations were 0（high glucose control group）,3.125,6.250,12.500,25.000 and 50.000mg·L^-1,meanwhile low glucose control group was set up.The cell proliferation was detected by MTT assay.The high glucose-treated mesangial cells were given GBE,total flavonoids,total ginkgolide,ginkgolide A,ginkgolide B,and ginkgolide C;the concentrations were 0（high glucose control group）,0.05,0.50,5.00,50.00mg·L^-1,meanwhile low glucose control group was set up.The levels of fibronectin（FN）and typeⅣ collagen（ColⅣ）were detected by ELISA method.Results The MTT results showed that compared with high glucose control group,the proliferation activity of mesangial cells in 50.00 mg·L^-1 GBE group was decreased significantly（P〈0.01）;the proliferation activities of mesangial cells in 25.00 and 50.00mg·L^-1 total flavonoids group were decreased significantly（P〈0.05 or P〈0.01）;the proliferation activities of mesangial cells in all quercetin,kaempferol,isorhamnetin and total flavonoids hydrolyzate groups were decreased significantly（P〈0.01）;the proliferation activity of mesganial cells in 50.00 mg·L^-1 ginkgolide C group was decreased significantly（P〈0.01）.The ELISA results showed that compared with high glucose control group,the levels of ColⅣ and FN in 5.00 and 50.00mg·L^-1 GBE groups were decreased significantly（P〈0.01）,and the FN level in0.50mg·L^-1 GBE group was decreased significantly（P〈0.05）;the ColⅣ levels in 0.50,5.00,and 50.00mg·L^-1 total flavonoids groups were decreased significantly（P〈0.05 or P〈0.01）;th

关 键 词：银杏叶提取物 糖尿病肾病 银杏总黄酮 银杏内酯 苷元 糖苷 

分 类 号：R587.2[医药卫生—内分泌]