解磷菌YC4对养殖环境条件的适应性及其溶磷效果  

Adaptability of phosphate solubilizing bacteria YC4 to aquaculture environment and its effect of phosphate solubilization

作　　者：杨莺莺[1] 杨铿[1] 梁晓华[1] 陈永青[1] 

机构地区：[1]中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《中国渔业质量与标准》2015年第4期23-28,共6页Chinese Fishery Quality and Standards

基　　金：中央级公益性科研院所基本科研业务费专项资金(2013YD09);现代农业(虾)产业技术体系建设专项资金(CARS-47);公益性行业(农业)科研专项(201103034)

摘　　要：YC4是从养殖池塘分离筛选的具解磷能力的芽孢杆菌，为给后续开发YC4菌肥提供基础资料，根据养殖池塘的环境条件，实验设置了不同温度、盐度、pH，测定YC4菌株在这些条件下48h时的菌量。结果表明，YC4在温度15～35℃，盐度0～35，pH6．0～10．0的范围内能生长；在温度20～35℃、盐度5～20、pH6．0～8．5条件下，YC4菌生长良好。因此，水产养殖环境条件适宜YC4的生长。本研究还考察YC4菌肥施用于养殖土塘后水体活性磷的变化情况，实验设计N＋P肥、YC4菌、YC4＋N肥、YC4＋N＋P肥等4种处理方法。结果表明，施用YC4菌或YC4菌肥组相比施用N＋P肥组的3组水体活性磷均增加，YC4＋N＋P及YC4组相比N＋P肥组水体活性磷最大增加值有显著性差异（P〈0．05），YC4菌的施用增加了水体的活性磷。本研究表明，YC4解磷菌具有在养殖土塘应用的潜力。YC4 is a phosphate solubilizing bacillus strain isolated from aquaculture ponds. In order to provide funda- mental information for developing a bacteria fertilizer from YC4, its adaptability to aquaculture pond environments was simulated by incubation under a wide range of water temperature, salinity and pH, and its abundance in 48 h was de- termined. The results showed that YC4 was able to grow under water temperature of 15 - 35 ℃, salinity of 0 - 35, and pH of 6.0 N 10.0, with optimal growth under temperature of 20 - 35 ℃ , salinity of 5 - 20 and pH of 6.0 - 8.5, suggesting its applicability under environmental conditions of aquaculture. To further examine the functioning of YC4 bacteria fertilizer, aquaculture earth ponds were treated with four combinations of the fertilizers, N ＋ P, YC4, YC4 ＋ N, and YC4 ＋ N ＋ P, and changes in dissolved inorganic phosphorus （DIP） were determined. The results showed that DIP increments were higher in the ponds treated with YC4, YC4 ＋ N or YC4 ＋ N ＋ P than in those controlled ponds treated with N ＋ P, and the DIP increments for YC4 ＋ N ＋ P and YC4 were statistically significantly （ P 〈 0.05 ）. The results suggested the important role of YC4 phosphate solubilizing bacillus in enhancing DIP and the po- tential of its application in aquaculture earth ponds.

关 键 词：解磷菌 环境条件 生长 活性磷 

分 类 号：S917.1[农业科学—水产科学]