抗脑损伤中药药效学的体外评价方法概述  

Study on evaluation method of pharmacodynamic of Chinese medicine in treating brain injury in vitro

作　　者：刘蕾[1] 赵晖[1] 王蕾[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《国际中医中药杂志》2015年第8期759-762,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81273742）;北京市属高等学校高层次人才引进与培养计划项目（CIT＆TCD20140329）

摘　　要：收集国内近10年抗脑损伤中药药效学体外实验研究方法的相关文献，并对其进行归纳和总结。研究发现，抗脑损伤中药药效学体外评价主要采用血清或脑脊液药理学的实验方法，常选用的细胞模型有β-淀粉样蛋白毒性模型、低糖低氧模型、1-甲基-4-苯基吡啶离子（MPP＋）毒性模型等，选用的评价指标有病理学、神经细胞凋亡、脑能量代谢及神经细胞氧化应激指标等。科研工作者对中药药效进行评价时，需根据实验目的确定恰当的实验模型和检测指标。The literatures on methods of pharmacodynamic of Chinese medicine in treating brain injury in vitro nearly I0 years were collected and summarized. The studies found that evaluated methods of pharmacodynamic of Chinese medicine in treating brain injury in vitro as followed： the experimental methods of anti-brain injury medicine were mainly used in vitro by serum or cercbrospinal fluid pharmacology, the cell models of β-amyloid toxicity, low sugar hypoxia, MPP＋ toxicity were often used, the indexes of evaluation such as pathology, neuronal apoptosis, brain energy metabolism and oxidative stress of neuronal cells were chosen. When the researchers evaluate the pharmacodynamic of Chinese medicine, the appropriate experimental models and indexes will be selected based on experimental purposes.

关 键 词：脑损伤 细胞模型 中药药效学评价指标 

分 类 号：R285[医药卫生—中药学]