膀胱感觉和运动神经支配的大鼠模型的建立及鉴定  

Establishment and Identification of Rat Model with Reconstruction of Bladder Sensory and Motor Innervation

作　　者：赵建国[1] 雷德桥 蒙德鹏[1] 侯春林[1] 林浩东[1] 宗海洋[1] 陈寅生[1] 蔡雨卫 

机构地区：[1]第二军医大学附属长征医院骨科,上海200003

出　　处：《中国临床医学》2015年第3期257-261,共5页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金资助项目(编号:10JC1418300)

摘　　要：目的:建立重建膀胱感觉和运动神经支配的SD大鼠模型并进行鉴定,为进一步研究排尿中枢重塑及其机制奠定基础。方法:45只雌性SD大鼠随机分为对照组(n=10)、神经根切断组(n=15)和神经根吻合组(n=20)。神经根切断组大鼠,将L4以下双侧脊神经前后根全部切断;神经根吻合组在切断脊神经根后,将双侧L4神经前后根与S1相应神经根吻合;对照组不作手术处理。术后6个月,取各组大鼠,分别行尿流动力学检测、神经根电刺激、神经吻合口甲苯胺蓝染色、盆神经节注射荧光金神经示踪染色和膀胱湿质量测量。结果:神经根吻合组大鼠膀胱最大容量、残余尿量、膀胱顺应性及膀胱湿质量均小于神经根切断组而大于对照组(P<0.05);神经根吻合组大鼠最大排尿压与对照组比较差异无统计学意义(P>0.05),但大于神经根切断组(P<0.05)。神经根吻合组电刺激神经根后膀胱内压升高,但低于对照组(P<0.05)。神经根吻合组神经吻合口甲苯胺蓝染色可见神经纤维通过率达(53.4±6.7)%。盆神经节内注射荧光金后,神经根吻合组可见L4脊髓节段双侧灰质荧光金染色,神经根切断组和对照组未在相应脊髓节段检测到荧光金染色。结论:成功建立了同时重建膀胱感觉及运动神经支配的大鼠动物模型,为进一步研究排尿中枢重塑及其机制奠定了基础。Objective：To establish and identify a SD rat model with reconstruction of bladder sensory and motor innervation,so as to lay the foundation for further study of micturition center remodeling and its mechanisms.Methods：A total of 45 female SD rats were randomly divided into control group（n=10）,rhizotomy group（n=15）and nerve root anastomosis group（n=20）.All the ventral and dorsal roots of spinal nerve below L4 level of rats in rhizotomy group were cut off.In nerve root anastomosis group,the bilateral ventral and dorsal roots of L4 nerve,were anastomosed with those of S1 nerve,after the spinal nerve roots had been cut off.Rats in control group were not treated with surgery.At Six months after surgery,rats in each group underwent urodynamic test,nerve root stimulation,toluidine blue staining at nerve anastomosis site,pelvic ganglia fluorescence gold tracer staining and bladder wet weight measurements.Results：Bladder maximum capacity,residual urine volume,bladder compliance and bladder wet weight in nerve root anastomosis group was less than those in rhizotomy group,however,larger than those in control group（P〈0.05）.There was no statistically significant difference in maximum voiding pressure between nerve root anastomosis group and control group（P〈0.05）,however,maximum voiding pressure in nerve root anastomosis group was larger than that in rhizotomy group（P〈0.05）.Intravesical pressure increased after nerve root stimulation in nerve root anastomosis group,but it was still lower than that in control group（P〈0.05）.Nerve passing rate was（53.4±6.7）%in nerve root anastomosis group,under toluidine blue staining at nerve anastomosis site.After injection of fluorescent gold in pelvic ganglia,fluorescence gold staining was visible in the L4 spinal cord gray matter in nerve root anastomosis group,however,not visible in rhizotomy group and control group.Conclusions：SD rat model with reconstruction of bladder sensory and motor innervation is successfully established.It lays the fo

关 键 词：尿流动力学 神经吻合 神经重塑 神经示踪 神经根电刺激 

分 类 号：R334.3[医药卫生—人体生理学]