河南宝天曼不同林龄与林型森林土壤的细菌群落结构与多样性  

Bacterial community structure and diversity in soils of different forest ages and types in Baotianman forest,Henan Province,China

作　　者：白晓旭[1,2] 史荣久[1] 尤业明[3] 盛华芳[4] 韩斯琴[1] 张颖[1] 

机构地区：[1]中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049 [3]北京林业大学森林培育与保护教育部重点实验室,北京100083 [4]南方医科大学公共卫生与热带医学学院环境卫生学系,广州510515

出　　处：《应用生态学报》2015年第8期2273-2281,共9页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(2011CB403205);国家自然科学基金重点项目(41330530)资助

摘　　要：采用高通量16S rRNA标签测序法,比较了地处北亚热带与暖温带过渡带的宝天曼自然保护区不同林龄与林分类型的土壤细菌群落结构及多样性.结果表明:宝天曼森林土壤细菌以变形菌门(29%)、酸杆菌门(18.5%)、疣微菌门(10%)等为主,共检测到60门1209属,优势属主要有疣微菌门的DA101(6.3%)、酸杆菌门的Acidobacteria-2(5.9%)和Candidatus Solibacter(2.9%)、泉古菌门的Candidatus Nitrososphaera(2.6%)等.不同林龄和林分类型土壤分别具有特有的种属组成及高丰度和低丰度种属.林龄与林分类型都对土壤微生物群落结构影响显著,且林分类型的影响大于林龄.80年林龄的锐齿栎土壤菌群多样性在不同林龄和林分类型中均最低.p H、土壤全氮、有机碳等是不同林龄及林分类型下土壤菌群结构变化的重要影响因子.To compare the microbial compositions and diversities in soils of different forest ages and types in Baotianman forest,Henan Province,China,genomic DNA of forest soils was extracted for amplifying the 16 S rRNA V4 hyper variable region by PCR and sequencing by Illumina Mi Seq. The BIPES,UCHIME and QIIME were employed to analyze the soil bacterial community. It was shown that 60 phyla were identified,with Proteobacteria,Acidobacteria,and Verrucomicrobia representing the most dominant lineages and accounting for 29%,18. 5% and 10% of all sequences,respectively. At the genus level,1209 genera were identified,the most abundant phylotypes were DA101（ 6. 3%）,Acidobacteria-2（ 5. 9%）,Candidatus Solibacter（ 2. 9%） and Candidatus Nitrososphaera（ 2. 6%）. Different forest age and type soil samples had unique compositions and specific high and rare genus. Forest type and age both impacted the soil microbial community structure,and the influence of the former was stronger than the latter. The soil microbial diversity of the 80-year-old Quercus aliena forest was the lowest among all age and type forest soil samples. Soil p H,soil nitrogen and organic carbon contents were the most important factors affecting soil bacterial community structure.

关 键 词：宝天曼暖温带森林 土壤微生物群落结构 16S rRNA ILLUMINA Miseq 

分 类 号：S714.3[农业科学—林学]