土元粪及浸提液对番茄根结线虫的防治作用  

Eupolyphaga frass and its extracts protected tomato from Meloidogyne incognita infestation

作　　者：王晓云[1] 王秀峰[1,2,3] 魏珉[1,2] 史庆华[1,2] 杨凤娟[1,2] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]作物生物学国家重点实验室,山东泰安271018 [3]农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安271018

出　　处：《应用生态学报》2015年第8期2511-2517,共7页Chinese Journal of Applied Ecology

基　　金：国家现代农业产业技术体系专项(CARS-25);"十二五"国家科技支撑计划项目(2014BAD05B03)资助

摘　　要：通过室内生测和盆栽试验,研究了土元粪及其浸提液对南方根结线虫的防治作用,并对土元粪有机挥发物成分及低聚壳聚糖进行了分析.结果表明:土元粪浸提液对根结线虫二龄幼虫具有击倒和致死作用,且随着浓度增加和时间延长而增加.浸提液的浓度≥20%具有明显的抑制线虫卵孵化的作用.盆栽试验表明,番茄根结线虫侵染形成的根结数量随土元粪使用量的增加而减少,相对防治效果随用量增加明显增大,且番茄株高、茎粗和叶片数随着土元粪用量的增加而增加.土元粪成分分析表明,土元粪中低聚壳聚糖含量达4.35%,有机挥发性物质有9大类110种.The control effects of Eupolyphaga（ Eupolyphaga sinensis Walker） frass and its extracts on Meloidogyne incognita were studied through laboratory assays and pot experiments,and the organic volatile compounds and oligochitosan contents in the frass were analyzed. The results indicated that the nematode immobility and mortality was significantly increased with increasing the extract concentration and treatment time. Compared with the control,egg hatching was significantly inhibited when the extract concentration was beyond 20%. Pot experiment indicated that root galling of tomato seedlings was reduced and the relative control effect was significantly improved with the increasing frass application. Meanwhile,plant height,stem diameter and leaf number of tomato increased with the increasing dosage of eupolyphaga frass. The ingredients analysis showed that the content of oligochitosan was about 4. 35% and there were 9 categories and 110 kinds of volatile compounds in the frass.

关 键 词：土元粪 浸提液 根结线虫 挥发性气体 壳聚糖 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]