厄贝沙坦联合金水宝对糖尿病肾病大鼠蛋白尿发生机制影响的研究  

Effects of the protection mechanism of podocyte in diabetic nephropathy rats of Irbesartan combined with Jinshuibao

作　　者：秦林燕[1] 王国兴[1] 

机构地区：[1]首都医科大学附属北京友谊医院急诊科,北京100050

出　　处：《临床和实验医学杂志》2015年第16期1313-1316,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:81374004)

摘　　要：目的研究厄贝沙坦联合金水宝对糖尿病肾病(DN)大鼠足细胞裂孔隔膜Podocin的表达影响,探讨DN蛋白尿的发生机制。方法建立糖尿病肾病大鼠动物模型。将动物随机分为厄贝组、金水宝组、联合组、糖尿病肾病组,另设正常对照组。12周后观察尿蛋白,用免疫组化、RT-PCR检测Podocin的表达,并观察Podocin与24尿蛋白的相关性。结果 1联合用药组较单用药组24 h尿蛋白量明显下降(P<0.05)。2联合用药组较单用药组Podocin蛋白表达、Podocin mRNA有明显升高(P<0.05)。3Podocin蛋白表达量和24 h尿蛋量具有显著负相关性(r=-0.889,P<0.001);而Podocin mRNA和24 h尿蛋白量具有显著正相关关系(r=0.933,P<0.001)。结论厄贝沙坦联合金水宝较单用药显著减轻糖尿病肾病大鼠早期蛋白尿,通过上调足细胞Podocin的表达,更好地保护肾小球滤过屏障。Objective To investigate the effects of podocin of kidney of early diabetic nephropathy（ DN）. To study the possible mechanism of proteinurin of diabetic nephropathy. Methods To construct diabetic nephropathy rats. After the successful finish model,the diabetes group was divided randomly into irbesartan group,Jinshuibao group,irbesartan combined with Jinshuibao group,diabetic nephropathy group,a normal group. After 12 weeks examination,the quantitation of proteinuria and biochemical indicator were measured and compared before the end of this study. To sacrifice all rats and the methods of immunohistochemistry and reverse transcriptase- PCR（ RT- PCR） were used to detect the expression of podocin of kidney tissue of all rats. And observe podocin relevance to 24 h urine protein. Results 1Compared single treatment group,24 hour proteinuria quantitation of combined treatment groups decreased markedly（ P 0. 05）. 2Compared single treatment group,the levels of relative podocin expression,podocin mRNA of combined treatment group increased markedly（ P 0. 05）. 3Podocin quantity of protein expression and 24 h urine protein have significant negative correlation（ r =- 0. 889,P 0. 001）. But Podocin mRNA and the 24 h urine protein have significant positive correlation（ r = 0. 933,P 0. 001）. Conclusion Irbesartan combined with Jinshuibao group compared with single treatment can up- regulation Podocin express of foot process glomerular membrane. Therefore,it can be relieve proteinuria of early diabetic nephropathy and protect glomerular filtration barrier.

关 键 词：大鼠 糖尿病肾病 厄贝沙坦 金水宝 蛋白尿 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]