5,5'-二硫代双(琥珀酰亚氨基-2-硝基苯甲酸)功能化的SERS纳米探针在免疫检验中的应用  

5,5'-Dithiobis( succinimidyl-2-nitrobenzoate) Functionalized Surface-enhanced Raman Scattering Nanoprobe for Immunoassay

作　　者：陈雷[1] 薛向欣[1] 崔运成[1] 韩晓霞[2] 徐蔚青[2] 赵冰[2] 

机构地区：[1]吉林师范大学环境友好材料制备与应用教育部重点实验室,四平136000 [2]吉林大学超分子结构与材料国家重点实验室,长春130012

出　　处：《高等学校化学学报》2015年第8期1505-1510,共6页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20150520015JH);国家自然科学基金(批准号:21403082;21273091);吉林师范大学博士科研启动项目(批准号:2014004)资助~~

摘　　要：以5,5'-二硫代双(琥珀酰亚氨基-2-硝基苯甲酸)(DSNB)分子作为偶联剂将蛋白质结合在金纳米粒子表面,既保持了蛋白质的生物活性,同时DSNB分子又具有较高的表面增强拉曼散射(SERS)活性,可作为蛋白质定量分析的探针分子.选用生物素与亲和素的特异性识别以及抗原抗体的免疫识别2个生物反应体系,将SERS纳米探针固定在蛋白质检测芯片上.以硅片为蛋白质检测载体,利用硅片在520 cm-1处的拉曼特征峰为内标,对人Ig G抗体进行定量分析.结果表明,该探针对人Ig G抗体检测的最低浓度可以达到5 pg/m L.A biofunctionalized nanoprobe for surface-enhanced Raman scattering（ SERS）-based immunoassay was designed. 5,5＇-Dithiobis（ succinimidyl-2-nitrobenzoate）（ DSNB） was employed as a linker for protein and gold nanoparticle,which can help to maintain the biological activity of the proteins. Besides that,DSNB possesses a high Raman scattering section,which can be used as the SERS probe as quantitative determination of proteins. Two kinds of biological systems（ avidin-streptavidin and antigen-antibody） were selected for the target protein and nanoprobe specific binding to the protein chip. Meanwhile,silicon slide was employed as a carrier,which provides a Raman peak at 520 cm^-1 and can be used as a good internal standard for quantitative analysis of proteins. Using this method,anti-human IgG concentration in solutions can be quantitatively measured at concentrations as low as 5 pg / mL.

关 键 词：表面增强拉曼散射 免疫检验 纳米探针 生物素 链霉亲和素 

分 类 号：O657[理学—分析化学]