头顶一颗珠提取液对大鼠脊髓损伤后CNTF及CNTFRα表达的影响  

Effect of extract of Trillium tschonoskii Maxim on ciliary neurotropic factor and its receptor α in rats suffering from spinal cord injury

作　　者：陈显兵[1] 朱旻玥 覃芙蓉[2] 唐尚权[2] 王凤杰[1] 龙波霖 袁德培[3] 

机构地区：[1]生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族学院医学院,湖北恩施445000 [3]湖北民族学院中医药学院,湖北恩施445000

出　　处：《解放军医学杂志》2015年第8期622-626,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81260521);湖北省自然科学基金(2014CFB613);生物资源保护与利用湖北省重点实验室基金(PKLHB1513)~~

摘　　要：目的观察百合科延龄草属植物头顶一颗珠干燥根茎提取液对大鼠急性脊髓损伤模型睫状神经营养因子(CNTF)及其受体(CNTFRα)表达的影响。方法健康成年Wistar大鼠45只,随机分为对照组、模型组及实验组(n=15),模型组及实验组参照Allen's法建立脊髓损伤模型,对照组不损伤脊髓。实验组于术前2周开始给予头顶一颗珠提取液灌胃,模型组和对照组给予同剂量蒸馏水灌胃。分别于伤后1d、7d,14d处死动物,取脊髓组织行HE染色观察组织病理学改变,Nissl染色观察Nissl小体的变化,并通过免疫组化染色、Western-blotting及RT-PCR检测观察CNTF、CNTFRαm RNA及蛋白的表达情况。结果 HE染色显示实验组脊髓结构比较清晰,神经细胞水肿、坏死较模型组减轻;Nissl染色见模型组和实验组损伤后各时相点脊髓前角运动神经元尼氏小体减少或消失,实验组尼氏小体减少程度较模型组明显减轻;免疫组化染色显示各组脊髓均有CNTF、CNTFRα蛋白表达。RT-PCR检测显示伤后7、14d模型组及实验组脊髓组织中CNTF、CNTFRαm RNA表达水平均明显高于对照组,且实验组明显高于模型组。Western blotting检测显示模型组伤后14d及实验组伤后7、14d脊髓组织中CNTF、CNTFRα蛋白表达水平明显高于对照组,且实验组伤后14d的表达水平均明显高于模型组。结论头顶一颗珠能上调脊髓组织中CNTF、CNTFRα的表达,对脊髓损伤有一定的保护作用。Objective To investigate the effect of Trillium tschonoskii Maxim extract on the expression of ciliary neurotrophic factor （CNTF） and its receptor （CNTFRα） after spinal cord injury in rats. Methods Forty-five rats were equally and randomly divided into control group （group A）, model group （group B） and Trillium tschonoskii Maxim treated group （group C）. Allen＇s weight drop method was used to reproduce acute spinal cord injury （SCI） model in rats of the group B and C. In group C, the rats were gavaged with Trillium tschonoslcii Maxim extract 2 weeks before the injury, while rats in group A and B were fed a same quantity of distilled water. 1, 7 or 14 days after injury, the rats were sacrificed to observe the structure of nerve cells after HE and Nissl staining, and the expression of CNTF and CNTFRα with immunohistochemical method, RT-PCR and Western blotting. Results HE staining showed that the structure of spinal cord in the the rats group C was more discernible, with milder edema and necrosis of nerve cells, as compared with that of group B. Nissl staining showed that Nissl bodies were decreased or disappeared in anterior horn motor neurons in both group B and C, but it was significantly less marked in group C than that in group B. Immunohistochemical staining, Western blotting and RT-PCR revealed that the protein and mRNA of CNTF and CNTFRα were positively expressed in rats of every group. The mRNA levels of CNTF and CNTFRα in group C were higher than those in group B.Conclusions Extract of Trillium tschonoskii Maxim can up-regulate the expression of CNTF and CNTFRα, and plays a protective role against injury to spinal cord.

关 键 词：脊髓损伤 睫状神经营养因子 延龄草 

分 类 号：R651.21[医药卫生—外科学]