高效液相色谱法测定养殖水体中的亚甲基蓝  

Determination of Methylene Blue in Aquaculture Water by HPLC

作　　者：龙举[1] 李子孟 喻亮[1] 李佩佩[1] 严忠雍[1] 张小军[1] 

机构地区：[1]浙江省海洋水产研究所浙江省海水增养殖重点实验室,浙江舟山316021

出　　处：《安徽农业科学》2015年第24期5-6,16,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省公益性技术研究项目(2014C32087);浙江科技计划项目(2013F10065)

摘　　要：[目的]探讨一种高效液相色谱法测定水产养殖水体环境中亚甲基蓝含量的分析方法，为亚甲基蓝测定方法的建立提供参考。[方法]将水样经预处理后利用二氯甲烷萃取，经旋转蒸发仪抽真空浓缩净化后高效液相色谱一紫外检测器检测，采用外标法定量分析。[结果]亚甲基蓝在0．05～1．00mg／L质量浓度范围内线性相关系数均大于0．999；在0．06-O．80mg／L添加范围内的平均回收率为75．2％～86．0％，相对标准偏差均小于12．0％；方法定量限为0．05mg/L。[结论]该方法的重现性较好，操作方便，定性准确且回收率较高，能够用于快速分析水产养殖水体环境中亚甲基蓝含量。[ Objective ] A high performance liquid chromatography analysis method for methylene blue in aquaculture water content was explored to provide reference for the establishment of methylene blue analysis method. [ Method]The water sample was extracted with dichloromethane af- ter the pretreatment,and concentrated and purified by a rotary evaporator pump vacuum and high performance liquid chromatography-UV detec- tor. Quantitative analysis was carried out with external standard method. [ Result] In the range of 0.05 - 1.00 mg/L,linear correlation coeffi- cients were all above 0.999. And In the ranges of 0.06 -0.80 mg/L,the mean fortification recoveries were 75.2% - 86.0%. RSDs were all less than 12.0% ,and quantitation limit was 0.05 mg/L. [ Conclusion] The proposed method has the advantages of good reproducibility, simplicity, qualitative accuracy and high recovery. And it can be successfully applied in the determination of methylene blue in aauaculture anuatic environment.

关 键 词：高效液相色谱 养殖水体 环境 亚甲基蓝 

分 类 号：S949[农业科学—水产养殖]