男性HIV／AIDS人群中梨支原体感染状况及其影响因素研究  

Study of Mycoplasma pirum infection and related factors among male HIV/AIDS patients

作　　者：陈璐斯[1] 吴建茹[1] 王蓓[1] 徐金水[2] 还锡萍[2] 

机构地区：[1]东南大学公共卫生学院,南京210009 [2]江苏省疾病预防控制中心

出　　处：《中华流行病学杂志》2015年第8期825-828,共4页Chinese Journal of Epidemiology

基　　金：教育部博士点基金（20130092110048）;国家自然科学基金（30872156）

摘　　要：目的了解江苏省男性HIV／AIDS人群中梨支原体（Mpi）感染情况，分析其感染的危险因素，并首次完成Mpi全基因组测序，为进一步研究其致病机制奠定基础。方法以江苏省疾病预防控制中心确认的男性HIV／AIDS人群为研究对象，采用重复横断面调查，进行了4次流行病学调查并分别收集首段尿、静脉血样本进行Mpi感染的检测和CD4＋T淋巴细胞计数。建立EpiData数据库，使用SPSS19．0软件进行统计学分析。采用IlluminaHiseq2000平台对Mpi基因组进行测序。结果共收集1541名男性HIV／AIDS，其中851人（55．2％）处于HIV感染阶段，690人（44．8％）已发展至艾滋病阶段，Mpi感染率15．4％（95％CI：14％～17％）；多因素logistic回归分析结果显示，在HIV／AIDS人群中未接受高效抗病毒治疗者，Mpi感染风险高于已治疗者（OR=1．344，95％CI：1．008～1．792）。另外，随CD4＋T淋巴细胞计数升高，脚i感染风险降低（OR=0．600，95％CI：0．444～0．810）。Mpi基因组为850704bp，GC含量为24．21％，含有708个基因，总长度为734085bp，平均长度1037bp，占基因组全长的86．29％。结论本次调查的1541名江苏省男性HIV／AIDS人群，Mpi感染较高。接受抗病毒治疗以及机体CD4＋T淋巴细胞计数保持较高水平者，可降低Mpi的感染风险。并首次完成Mpi标准株的全基因组序列测定（注册序列号：AZHZ00000001），获得了基因组草图，为今后进一步研究基因功能、探求致病机制等提供依据。Objective To investigate the infection status of Mycoplasma pirurn among male HIV/AIDS patients in Jiangsu and analyze the risk factors. The genome sequencing of Mycoplasma pirum was completed for the first time. Methods Male HIV infected individuals and AIDS patients confirmed in Jiangsu province were enrolled for 4 repeated cross-sectional studies by means of detecting the first flow urine sample and venous blood sample collected and questionnaire survey after informed consent. Genome sequencing was conducted for Mycoplasma pirurn by using Illumina Hiseq 2000 sequencing platform. Results A total of 1 541 HIV/AIDS patients were surveyed in this study. The infection rates of Mycoplasma pirum was 15.4%. The patients who received no HAART had higher risk to be infected with Mycoplasma pirum （OR= 1.344, 95% CI： 1.008-1.792）. Otherwise, high CD4＋T counts was a protective factor for Mycoplasma pirum infection （OR=0.600, 95%CI： 0.444-0.810）. Based on the sequencing result, the genome size of Mycoplasma pirum was 850 704 bp, the GC content was 24.21% the genome contained 708 genes, the total length of genes was 734 085 bp, the average length was 1 037 bp, accounting for 86.29% of genome. Conclusion More attention should be paid to the high infection rate of Mycoplasma pirum amongmale HIV/AIDS patients in the future AIDS prevention and control. The first genome sequencing of standard Mycoplasma pirum strain was completed in this study （registering serial mmaber： AZHZ00000001） , which can provide evidence for the further research of gene function and pathogenic mechanism of Mycoplasma pirum.

关 键 词：梨支原体 HIV感染者/艾滋病患者 感染率 全基因组 

分 类 号：R512.91[医药卫生—内科学]